NDLI: Metabolic engineering of Clostridium acetobutylicum for enhanced production of butyric acid

Content Provider	Springer Nature Link
Author	Jang, Yu Sin Woo, Hee Moon Im, Jung Ae Kim, In Ho Lee, Sang Yup
Copyright Year	2013
Abstract	Clostridium acetobutylicum has been considered as an attractive platform host for biorefinery due to its metabolic diversity. Considering its capability to overproduce butanol through butyrate, it was thought that butyric acid can also be efficiently produced by this bacterium through metabolic engineering. The pta-ctfB-deficient C. acetobutylicum CEKW, in which genes encoding phosphotransacetylase and CoA-transferase were knocked out, was assessed for its potential as a butyric acid producer in fermentations with four controlled pH values at 5.0, 5.5, 6.0, and 6.4. Butyric acid could be best produced by fermentation of the CEKW at pH 6.0, resulting in the highest titer of 26.6 g/l, which is 6.4 times higher than that obtained with the wild type. However, due to the remaining solventogenic ability of the CEKW, 3.6 g/l solvents were also produced. Thus, the CEKW was further engineered by knocking out the adhE1-encoding aldehyde/alcohol dehydrogenase to prevent solvent production. Batch fermentation of the resulting C. acetobutylicum HCEKW at pH 6.0 showed increased butyric acid production to 30.8 g/l with a ratio of butyric-to-acetic acid (BA/AA) of 6.6 g/g and a productivity of 0.72 g/l/h from 86.9 g/l glucose, while negligible solvent (0.8 g/l ethanol only) was produced. The butyric acid titer, BA/AA ratio, and productivity obtained in this study were the highest values reported for C. acetobutylicum, and the BA/AA ratio and productivity were also comparable to those of native butyric acid producer Clostridium tyrobutyricum. These results suggested that the simultaneous deletion of the pta-ctfB-adhE1 in C. acetobutylicum resulted in metabolic switch from biphasic to acidogenic fermentation, which enhanced butyric acid production.
Starting Page	9355
Ending Page	9363
Page Count	9
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	97
Issue Number	21
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-09-08
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Butyric acid BA/AA ratio Clostridium acetobutylicum Metabolic engineering Knockout Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Engineering Clostridium acetobutylicum with a histidine kinase knockout for enhanced n-butanol tolerance and production

New insights into the butyric acid metabolism of Clostridium acetobutylicum

Metabolic engineering of Candida utilis for isopropanol production

Application of new metabolic engineering tools for Clostridium acetobutylicum

Production of isopropanol by metabolically engineered Escherichia coli

Assessment of heterologous butyrate and butanol pathway activity by measurement of intracellular pathway intermediates in recombinant Escherichia coli

Roles of three AbrBs in regulating two-phase Clostridium acetobutylicum fermentation

Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli

Phosphoproteomic investigation of a solvent producing bacterium Clostridium acetobutylicum

Metabolic engineering of Clostridium acetobutylicum for enhanced production of butyric acid

Similar Documents

Engineering Clostridium acetobutylicum with a histidine kinase knockout for enhanced n-butanol tolerance and production

New insights into the butyric acid metabolism of Clostridium acetobutylicum

Metabolic engineering of Candida utilis for isopropanol production

Application of new metabolic engineering tools for Clostridium acetobutylicum

Production of isopropanol by metabolically engineered Escherichia coli

Assessment of heterologous butyrate and butanol pathway activity by measurement of intracellular pathway intermediates in recombinant Escherichia coli

Roles of three AbrBs in regulating two-phase Clostridium acetobutylicum fermentation

Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli

Phosphoproteomic investigation of a solvent producing bacterium Clostridium acetobutylicum

Metabolic engineering of Clostridium acetobutylicum for enhanced production of butyric acid