NDLI: Fusion of agarase and neoagarobiose hydrolase for mono-sugar production from agar

Content Provider	Springer Nature Link
Author	Alkotaini, Bassam Han, Nam Soo Kim, Beom Soo
Copyright Year	2016
Abstract	In enzymatic saccharification of agar, endo- and exo-agarases together with neoagarobiose hydrolase (NABH) are important key enzymes for the sequential hydrolysis reactions. In this study, a bifunctional endo/exo-agarase was fused with NABH for production of mono-sugars (d-galactose and 3,6-anhydro-l-galactose) from agar using only one fusion enzyme. Two fusion enzymes with either bifunctional agarase (Sco3476) or NABH (Zg4663) at the N-terminus, Sco3476–Zg4663 (SZ) and Zg4663–Sco3476 (ZS), were constructed. Both fusion enzymes exhibited their optimal agarase and NABH activities at 40 and 35 °C, respectively. Fusions SZ and ZS enhanced the thermostability of the NABH activity, while only fusion SZ showed a slight enhancement in the NABH catalytic efficiency (K cat/K M) from 14.8 (mg/mL)−1 s−1 to 15.8 (mg/mL)−1 s−1. Saccharification of agar using fusion SZ resulted in 2-fold higher mono-sugar production and 3-fold lower neoagarobiose accumulation when compared to the physical mixture of Sco3476 and Zg4663. Therefore, this fusion has the potential to reduce enzyme production cost, decrease intermediate accumulation, and increase mono-sugar yield in agar saccharification.
Starting Page	1573
Ending Page	1580
Page Count	8
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	101
Issue Number	4
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-11-25
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Bifunctional agarase Neoagarobiose hydrolase Fusion enzyme Agar saccharification Red algae Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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