NDLI: Secretion of Kluyveromyces lactis Cu/Zn SOD: strategies for enhanced production

Content Provider	Springer Nature Link
Author	Raimondi, Stefa Uccelletti, Daniela Amaretti, Alberto Leonardi, Alan Palleschi, Claudio Rossi, Maddalena
Copyright Year	2009
Abstract	The Kluyveromyces lactis Cu/Zn SOD gene (SOD1) was fused with the toxin K1 signal sequence to obtain extracellular production of superoxide dismutase. Kluyveromyces marxianus L3 and K. lactis MW98-8C strains were transformed and compared as hosts for the secretion. The effects of the media composition were evaluated: In K. lactis, the highest volumetric activity was obtained in YKK synthetic medium in the presence of Cu2+/Zn2+ cofactors (9.6 kU l−1). In K. marxianus, active SOD was produced only in YPD medium supplemented with Cu2+ and Zn2+ (8.8 kU l−1). In order to improve the production of secreted active SOD in K. lactis, the SOD1 copper carrier (CCS1) was overexpressed and targeted to the secretory apparatus. A positive effect was observed only when K. lactis was grown in a medium without Cu2+/Zn2+ supplement. The best performing culture conditions for K. lactis and K. marxianus recombinant strains were successfully applied to two laboratory-scale fed-batch processes, and volumetric SOD activities increased up to 19.4 and 24.1 kU l−1, respectively.
Starting Page	871
Ending Page	878
Page Count	8
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	86
Issue Number	3
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-12-10
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Kuyveromyces marxianus Kluyveromyces lactis Superoxide dismutase Proteins secretion Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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