NDLI: Occurrence of lignin degradation genotypes and phenotypes among prokaryotes

Content Provider	Springer Nature Link
Author	Tian, Jiang Hao Pourcher, Anne Marie Bouchez, Théodore Gelhaye, Eric Peu, Pascal
Copyright Year	2014
Abstract	A number of prokaryotes actively contribute to lignin degradation in nature and their activity could be of interest for many applications including the production of biogas/biofuel from lignocellulosic biomass and biopulping. This review compares the reliability and efficiency of the culture-dependent screening methods currently used for the isolation of ligninolytic prokaryotes. Isolated prokaryotes exhibiting lignin-degrading potential are presented according to their phylogenetic groups. With the development of bioinformatics, culture-independent techniques are emerging that allow larger-scale data mining for ligninolytic prokaryotic functions but today, these techniques still have some limits. In this work, two phylogenetic affiliations of isolated prokaryotes exhibiting ligninolytic potential and laccase-encoding prokaryotes were determined on the basis of 16S rDNA sequences, providing a comparative view of results obtained by the two types of screening techniques. The combination of laboratory culture and bioinformatics approaches is a promising way to explore lignin-degrading prokaryotes.
Starting Page	9527
Ending Page	9544
Page Count	18
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	98
Issue Number	23
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-10-26
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Lignin Degradation Prokaryotes Screening Culture-dependent Culture-independent Phylogenetic distribution Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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