NDLI: Production of triacylglycerols in Escherichia coli by deletion of the diacylglycerol kinase gene and heterologous overexpression of atfA from Acinetobacter baylyi ADP1

Content Provider	Springer Nature Link
Author	Janßen, Helge Jans Steinbüchel, Alexander
Copyright Year	2014
Abstract	This study investigated the production of triacylglycerols in cells of the wild type of Escherichia coli and of a strain with a deleted diacylglycerol kinase gene (dgkA). By overexpression of atfA from Acinetobacter baylyi ADP1 and fadD from E. coli in the dgkA deletion mutant, cellular contents of up to 4.9 % (w/w) triacylglycerols could be achieved in batch cultivation. Furthermore, heterologous expression of atfA relieves the negative effects of dgkA deletion on growth. Process optimization and fed-batch fermentation resulted in the production of 530 mg l −1 triacylglycerols and a maximal content of 8.5 % (w/w) triacylglycerols of the cell dry mass. This clearly exceeded all previous results concerning triacylglycerol production in E. coli. Furthermore, the production of extracellular free fatty acids and fatty acid ethyl esters was investigated. Like triacylglycerols, both products are potential biofuels, and we show their continuous production in a repeated batch process, with recovery of the production cells.
Starting Page	1913
Ending Page	1924
Page Count	12
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	98
Issue Number	4
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-01-05
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Biofuels Diacylglycerol Escherichia coli Lipids Triacylglycerol Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Improved Triacylglycerol Production in Acinetobacter baylyi ADP1 by Metabolic Engineering

RESEARCH Open Access Improved Triacylglycerol Production in Acinetobacter baylyi ADP1 by Metabolic

Improved Triacylglycerol Production in Acinetobacter baylyi ADP1 by Metabolic Engineering

Improving heterologous polyketide production in Escherichia coli by overexpression of an S-adenosylmethionine synthetase gene

Overexpression of a phosphatidic acid phosphatase type 2 leads to an increase in triacylglycerol production in oleaginous Rhodococcus strains

Improving heterologous polyketide production in Escherichia coli by transporter engineering

Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology

Impact of deletion of the genes encoding acetate kinase on production of L-tryptophan by Escherichia coli

Effect of folding factors in rescuing unstable heterologous lipase B to enhance its overexpression in the periplasm of Escherichia coli

Production of triacylglycerols in Escherichia coli by deletion of the diacylglycerol kinase gene and heterologous overexpression of atfA from Acinetobacter baylyi ADP1

Similar Documents

Improved Triacylglycerol Production in Acinetobacter baylyi ADP1 by Metabolic Engineering

RESEARCH Open Access Improved Triacylglycerol Production in Acinetobacter baylyi ADP1 by Metabolic

Improved Triacylglycerol Production in Acinetobacter baylyi ADP1 by Metabolic Engineering

Improving heterologous polyketide production in Escherichia coli by overexpression of an S-adenosylmethionine synthetase gene

Overexpression of a phosphatidic acid phosphatase type 2 leads to an increase in triacylglycerol production in oleaginous Rhodococcus strains

Improving heterologous polyketide production in Escherichia coli by transporter engineering

Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology

Impact of deletion of the genes encoding acetate kinase on production of L-tryptophan by Escherichia coli

Effect of folding factors in rescuing unstable heterologous lipase B to enhance its overexpression in the periplasm of Escherichia coli

Production of triacylglycerols in Escherichia coli by deletion of the diacylglycerol kinase gene and heterologous overexpression of atfA from Acinetobacter baylyi ADP1