NDLI: Effect of dilution rate, cellobiose and ammonium availabilities on Clostridium cellulolyticum sporulation

Content Provider	Springer Nature Link
Author	Payot, S. Guedon, E. Desvaux, M. Gelhaye, E. Petitdemange, E.
Copyright Year	1999
Abstract	The nutritional and physiological factors affecting sporulation of Clostridium cellulolyticum were studied using steady-state continuous cultures grown in both complex and synthetic media. Under cellobiose limitation, the probability that cells will sporulate appears to be directly related to the growth rate. In complex medium, the highest percentage of sporulation was 20% at a dilution rate of 0.015 h−1 whereas in synthetic medium it was 10% at 0.035 h−1. In both media, when the dilution rate was either higher or lower the percentage of sporulation decreased by between 2% and 4%. At low dilution rates, endospore formation was repressed under cellobiose-sufficient concentrations, suggesting catabolite repression by cellobiose. Furthermore, the concentration of ammonium was important in determining the percentage of sporulation, as ammonium limitation induced extensive sporulation at low growth rates even in an excess of cellobiose. The sporulation process is not triggered when cells are cellobiose-exhausted both in complex and synthetic media. These data suggest that, in C. cellulolyticum, an exogenous supply of carbon is required throughout the sporulation process. In the experimental conditions used in this work, no relationship between glycogen accumulation or glycogen mobilization and endospore formation was detected in C. cellulolyticum.
Starting Page	670
Ending Page	674
Page Count	5
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	52
Issue Number	5
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-11-03
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Clostridium cellulolyticum Viability and Sporulation under Cellobiose Starvation Conditions

Improvement of cellulose catabolism in Clostridium cellulolyticum by sporulation abolishment and carbon alleviation

Electrotransformation studies in Clostridium cellulolyticum

Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system

Relationships between cellobiose catabolism, enzyme levels, and metabolic intermediates inClostridium cellulolyticum grown in a synthetic medium

Metabolic engineering of Clostridium cellulolyticum for the production of n-butanol from crystalline cellulose

Effect of dilution rate on the microbial structure of a mesophilic butyrate-degrading methanogenic community during continuous cultivation

Flux Analysis of the Metabolism of Clostridium cellulolyticum Grown in Cellulose-Fed Continuous Culture on a Chemically Defined Medium under Ammonium-Limited Conditions

Studies of Clostridium cellulolyticum ATCC 35319 under dialysis and co-culture conditions

Effect of dilution rate, cellobiose and ammonium availabilities on Clostridium cellulolyticum sporulation

Similar Documents

Clostridium cellulolyticum Viability and Sporulation under Cellobiose Starvation Conditions

Improvement of cellulose catabolism in Clostridium cellulolyticum by sporulation abolishment and carbon alleviation

Electrotransformation studies in Clostridium cellulolyticum

Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system

Relationships between cellobiose catabolism, enzyme levels, and metabolic intermediates inClostridium cellulolyticum grown in a synthetic medium

Metabolic engineering of Clostridium cellulolyticum for the production of n-butanol from crystalline cellulose

Effect of dilution rate on the microbial structure of a mesophilic butyrate-degrading methanogenic community during continuous cultivation

Flux Analysis of the Metabolism of Clostridium cellulolyticum Grown in Cellulose-Fed Continuous Culture on a Chemically Defined Medium under Ammonium-Limited Conditions

Studies of Clostridium cellulolyticum ATCC 35319 under dialysis and co-culture conditions

Effect of dilution rate, cellobiose and ammonium availabilities on Clostridium cellulolyticum sporulation