NDLI: Microbial diversity observed during hemp retting

Content Provider	Springer Nature Link
Author	Ribeiro, Alexandra Pochart, Philippe Day, Arnaud Mennuni, Sarah Bo, Pierre Baret, Jean Luc Spadoni, Jean Louis Mangin, Irène
Copyright Year	2015
Abstract	Historically used in textile and paper industry, hemp fibres have started to find new applications in composite materials with important economic and ecological advantages. However, their applications are limited since manufacturers have some difficulties to standardise fabrication processes. This study is a first step before selection and isolation of strains that could later be used to optimise microbial retting efficiency and hence fibre quality. We studied six samples harvested on different ground types, at different dates and with different retting durations on field to obtain an exhaustive representation of the process. After DNA extraction, total bacteria and fungi associated with stems during retting were specifically quantified using real-time PCR. Then, using sequence analysis of randomly cloned 16S and 18S ribosomal RNA (rRNA) genes, a phylogenetic characterisation of the dominant microorganisms was carried out. Quantitatively, we showed that there were 8.1–9.5 log10 16S rRNA gene copies per gram of hemp straw for bacteria and 8.6–9.6 log10 18S rRNA gene copies per gram for fungi. Qualitatively, we noticed a higher bacterial diversity in comparison to fungi. This work showed that in the different samples, the same species were present but in significantly different proportions according to ground type, harvest dates and retting durations on field. The most frequent bacterial sequences were affiliated to species Escherichia coli, Pantoea agglomerans, Pseudomonas rhizosphaerae, Rhodobacter sp., Pseudomonas fulva, Rhizobium huautlense and Massilia timonae, whereas fungal sequences were principally related to the genera Cladosporium and Cryptococcus.
Starting Page	4471
Ending Page	4484
Page Count	14
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	99
Issue Number	10
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-01-11
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Hemp retting Microbiota Molecular inventory qPCR Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterisation of a new thermoalkaliphilic bacterium for the production of high-quality hemp fibres, Geobacillus thermoglucosidasius strain PB94A

Recovery of microbial diversity and activity during bioremediation following chemical oxidation of diesel contaminated soils

The gastrointestinal tract microbiota of the Japanese quail, Coturnix japonica

Inventory and monitoring of wine microbial consortia

Diversity of the resident microbiota in a thermophilic municipal biogas plant

Estimating the bias related to DNA recovery from hemp stems for retting microbial community investigation

Enhancement of the microbial community biomass and diversity during air sparging bioremediation of a soil highly contaminated with kerosene and BTEX

International Space Station environmental microbiome — microbial inventories of ISS filter debris

Assess the diversity of gut microbiota among healthy adults for forensic application

Microbial diversity observed during hemp retting

Similar Documents

Characterisation of a new thermoalkaliphilic bacterium for the production of high-quality hemp fibres, Geobacillus thermoglucosidasius strain PB94A

Recovery of microbial diversity and activity during bioremediation following chemical oxidation of diesel contaminated soils

The gastrointestinal tract microbiota of the Japanese quail, Coturnix japonica

Inventory and monitoring of wine microbial consortia

Diversity of the resident microbiota in a thermophilic municipal biogas plant

Estimating the bias related to DNA recovery from hemp stems for retting microbial community investigation

Enhancement of the microbial community biomass and diversity during air sparging bioremediation of a soil highly contaminated with kerosene and BTEX

International Space Station environmental microbiome — microbial inventories of ISS filter debris

Assess the diversity of gut microbiota among healthy adults for forensic application

Microbial diversity observed during hemp retting