NDLI: Two non-exclusive strategies employed to protect Torulopsis glabrata against hyperosmotic stress

Content Provider	Springer Nature Link
Author	Yu, Xiaoxia Wang, Hongjiang Liu, Liming
Copyright Year	2014
Abstract	Several recent reports described an apoptosis-like programmed cell death (PCD) process in yeast in response to different environmental challenges. In this study, hyperosmotic stress caused by high NaCl concentration in culture medium induced cell death in the haploid yeast Torulopsis glabrata. Propidium iodide (PI) and PI/rhodamine-123 (Rh123) dual staining with flow cytometry showed that high salinity decreased intact cells by 16.5 %, increased necrotic cells by nearly twofold, and altered fermentative parameters appreciably. Morphological and biochemical indicators of apoptosis were apparent, specifically a decrease in mitochondrial membrane potential (∆Ψm), translocation of phosphatidylserine (PS) from the inner to the outer side of the plasma membrane, generation of reactive oxygen species (ROS), and involvement of caspase all while plasma membrane integrity was maintained. Additionally, it was found that overexpression of YCA1 drastically stimulated cell death, indicating that activation of metacaspase might lead to cell death. However, T. glabrata growth under hyperosmotic stress was enhanced when FIS1, HOG1, and GPD2 were overexpressed, or when exogenous proline or glutathione (GSH) were added into the cultures, both of which could repress caspase-3 activity. Thus, in these concrete cases of overexpression of anti-apoptotic or anti-necrotic factors and pharmacological manipulations, it decreased T. glabrata cell death that might help to achieve higher fermentative efficiency.
Starting Page	3099
Ending Page	3110
Page Count	12
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	98
Issue Number	7
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-02-25
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Torulopsis glabrata Apoptosis Hyperosmotic stress Exogenous/endogenous intervention Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Redirection of the NADH oxidation pathway in Torulopsis glabrata leads to an enhanced pyruvate production

Proline enhances Torulopsis glabrata growth during hyperosmotic stress

Microbial response to environmental stresses: from fundamental mechanisms to practical applications

Water-forming NADH oxidase protects Torulopsis glabrata against hyperosmotic stress.

Anaerobic glycerol production by Saccharomyces cerevisiae strains under hyperosmotic stress

Identification of a polysaccharide produced by the pyruvate overproducer Candida glabrata CCTCC M202019

Biotin enhances salt tolerance of Torulopsis mogii

Calcium and oxidative stress mediate perillaldehyde-induced apoptosis in Candida albicans

Enhancement of pyruvate production by Torulopsis glabrata using a two-stage oxygen supply control strategy

Two non-exclusive strategies employed to protect Torulopsis glabrata against hyperosmotic stress

Similar Documents

Redirection of the NADH oxidation pathway in Torulopsis glabrata leads to an enhanced pyruvate production

Proline enhances Torulopsis glabrata growth during hyperosmotic stress

Microbial response to environmental stresses: from fundamental mechanisms to practical applications

Water-forming NADH oxidase protects Torulopsis glabrata against hyperosmotic stress.

Anaerobic glycerol production by Saccharomyces cerevisiae strains under hyperosmotic stress

Identification of a polysaccharide produced by the pyruvate overproducer Candida glabrata CCTCC M202019

Biotin enhances salt tolerance of Torulopsis mogii

Calcium and oxidative stress mediate perillaldehyde-induced apoptosis in Candida albicans

Enhancement of pyruvate production by Torulopsis glabrata using a two-stage oxygen supply control strategy

Two non-exclusive strategies employed to protect Torulopsis glabrata against hyperosmotic stress