NDLI: Fermentation and metabolic characteristics of Gluconacetobacter oboediens for different carbon sources

Content Provider	Springer Nature Link
Author	Sarkar, Dayanidhi Yabusaki, Masahiro Hasebe, Yuta Ho, Pei Yee Kohmoto, Shuji Kaga, Takayuki Shimizu, Kazuyuki
Copyright Year	2010
Abstract	The metabolism of Gluconacetobacter oboediens was investigated in relation to different carbon sources for the continuous cultures at the dilution rate of 0.05 h−1. The 13C-flux result implies the formation of metabolic recycles for the case of using glucose and acetate as carbon sources. When glucose and ethanol were used as carbon sources, the specific ethanol uptake rate and the specific acetate production rate increased as the feed ethanol concentration was increased from 40 to 60 g/l, while the specific CO2 production rate and the biomass concentration decreased, where the 13C-metabolic flux result indicates that the glycolysis, oxidative PP pathway, and the tricarboxylic acid (TCA) cycle were less active, resulting in less biomass concentration. The flux result also implies that oxaloacetate decarboxylase flux became negative, so that oxaloacetate is backed up by this pathway, resulting in less activity of glyoxylate pathway. When gluconate was added for the case of using glucose and ethanol as carbon sources, the acetate and cell concentrations as well as gluconate concentrations increased. The glucose and ethanol concentrations decreased concomitantly with the increased feed gluconate concentration. In accordance with these fermentation characteristics, the enzyme activity result indicates that glucose dehydrogenase and glucose-6-phosphate dehydrogenase pathways became less active, while the glycolysis and the TCA cycle was activated as the feed gluconate concentration was increased.
Starting Page	127
Ending Page	136
Page Count	10
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	87
Issue Number	1
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-02-27
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Gluconacetobacter oboediens Chemostat culture Enzyme activity 13C-labeling experiment Metabolic flux analysis Acetate fermentation Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The chemostat study of metabolic distribution in extreme-thermophilic (70 °C) mixed culture fermentation

Metabolic flux analysis of Gluconacetobacter xylinus for bacterial cellulose production

The benefits of being transient: isotope-based metabolic flux analysis at the short time scale

Metabolic fluxes in Schizosaccharomyces pombe grown on glucose and mixtures of glycerol and acetate

Physiological characterization of recombinant Saccharomyces cerevisiae expressing the Aspergillus nidulans phosphoketolase pathway: validation of activity through 13C-based metabolic flux analysis

Metabolic flux distributions: genetic information, computational predictions, and experimental validation

Characterization of microbial compositions in a thermophilic chemostat of mixed culture fermentation

Optimization of culture conditions for bacterial cellulose production from Gluconacetobacter hansenii UAC09

A chemostat study of Streptomyces peucetius var. caesius N47

Fermentation and metabolic characteristics of Gluconacetobacter oboediens for different carbon sources

Similar Documents

The chemostat study of metabolic distribution in extreme-thermophilic (70 °C) mixed culture fermentation

Metabolic flux analysis of Gluconacetobacter xylinus for bacterial cellulose production

The benefits of being transient: isotope-based metabolic flux analysis at the short time scale

Metabolic fluxes in Schizosaccharomyces pombe grown on glucose and mixtures of glycerol and acetate

Physiological characterization of recombinant Saccharomyces cerevisiae expressing the Aspergillus nidulans phosphoketolase pathway: validation of activity through 13C-based metabolic flux analysis

Metabolic flux distributions: genetic information, computational predictions, and experimental validation

Characterization of microbial compositions in a thermophilic chemostat of mixed culture fermentation

Optimization of culture conditions for bacterial cellulose production from Gluconacetobacter hansenii UAC09

A chemostat study of Streptomyces peucetius var. caesius N47

Fermentation and metabolic characteristics of Gluconacetobacter oboediens for different carbon sources