NDLI: Glucose exerts a negative effect over a peroxidase from Trichosporon asahii, with carotenoid cleaving activity

Content Provider	Springer Nature Link
Author	Rodríguez Bustamante, Eduardo Maldonado Robledo, Gabriela Arreguín Espisa, Roberto Mendoza Hernández, Guillermo Rodríguez Saja, Romina Sánchez, Sergio
Copyright Year	2009
Abstract	Tobacco aroma compounds were generated via lutein cleavage by the combined action of a yeast and a bacterium identified as Trichosporon asahii and Paenibacillus amylolyticus, respectively. In this study, an inverse relationship between glucose concentration and the generation of three compounds, present in the tobacco aroma profile, was observed in mixed cultures. In order to identify the organism sensitive to the sugar effect, both were grown separately. The presence of glucose suppressed β-ionone production by T. asahii grown with lutein. However, the biotransformation of the ionone into its reduced derivatives (7,8-dihydro-β-ionone and 7,8-dihydro-β-ionol) by P. amylolyticus was not affected by the sugar . This pointed to the cleavage of lutein, a step within the process necessary for the synthesis of β-ionone, as the target of the glucose effect. In vitro studies with crude extracts and concentrated cell-free medium derived from T. asahii cultures showed that the carotenoid breakdown activity was located extracellularly and only detected in supernatants from yeast cells grown in the absence of the sugar. Rather than an inhibition or a mechanism affecting the enzyme secretion, the glucose effect on lutein degradation comprised another regulatory level. Further experiments showed that the enzyme responsible for lutein breakdown and susceptible to the sugar effect exhibited a high degree of identity to fungal peroxidases, studied as well, for their involvement in carotenoid cleavage.
Starting Page	499
Ending Page	510
Page Count	12
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	84
Issue Number	3
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-04-24
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Glucose negative effect Lutein cleavage β-ionone and its reduced derivatives Fungal peroxidases Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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