NDLI: Porcine major histocompatibility complex (MHC) class I molecules and analysis of their peptide-binding specificities

Content Provider	Springer Nature Link
Author	Pedersen, Lasse Eggers Harndahl, Mikkel Rasmussen, Michael Lamberth, Kasper Golde, William T. Lund, Ole Nielsen, Morten Buus, Soren
Copyright Year	2011
Abstract	In all vertebrate animals, CD8+ cytotoxic T lymphocytes (CTLs) are controlled by major histocompatibility complex class I (MHC-I) molecules. These are highly polymorphic peptide receptors selecting and presenting endogenously derived epitopes to circulating CTLs. The polymorphism of the MHC effectively individualizes the immune response of each member of the species. We have recently developed efficient methods to generate recombinant human MHC-I (also known as human leukocyte antigen class I, HLA-I) molecules, accompanying peptide-binding assays and predictors, and HLA tetramers for specific CTL staining and manipulation. This has enabled a complete mapping of all HLA-I specificities (“the Human MHC Project”). Here, we demonstrate that these approaches can be applied to other species. We systematically transferred domains of the frequently expressed swine MHC-I molecule, SLA-10401, onto a HLA-I molecule (HLA-A11:01), thereby generating recombinant human/swine chimeric MHC-I molecules as well as the intact SLA-10401 molecule. Biochemical peptide-binding assays and positional scanning combinatorial peptide libraries were used to analyze the peptide-binding motifs of these molecules. A pan-specific predictor of peptide–MHC-I binding, NetMHCpan, which was originally developed to cover the binding specificities of all known HLA-I molecules, was successfully used to predict the specificities of the SLA-10401 molecule as well as the porcine/human chimeric MHC-I molecules. These data indicate that it is possible to extend the biochemical and bioinformatics tools of the Human MHC Project to other vertebrate species.
Starting Page	821
Ending Page	834
Page Count	14
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	63
Issue Number	12
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-07-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Recombinant MHC Peptide specificity Binding predictions Cell Biology Human Genetics Gene Function Immunology Allergology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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