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Expressed antibody repertoires in human cord blood cells: 454 sequencing and IMGT/HighV-QUEST analysis of germline gene usage, junctional diversity, and somatic mutations

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Expressed antibody repertoires in human cord blood cells: 454 sequencing and IMGT/HighV-QUEST analysis of germline gene usage, junctional diversity, and somatic mutations

Content Provider	Springer Nature Link
Author	Prabakaran, Ponraj Chen, Weizao Singarayan, Maria G. Stewart, Claudia C. Streaker, Emily Feng, Yang Dimitrov, Dimiter S.
Copyright Year	2011
Abstract	Human cord blood cell-derived IgM antibodies are important for the neonate immune responses and construction of germline-based immunoglobulin libraries. Several previous studies of a relatively small number of sequences found that they exhibit restrictions in the usage of germline genes and in the diversity of the variable heavy chain complementarity determining region 3 compared to adults. To further characterize such restrictions on a larger scale and to compare the early B-cell diversity to adult IgM repertoires, we performed 454 sequencing and IMGT/HighV-QUEST analysis of cord blood IG libraries from two babies and determined germline gene usage, V-D-J rearrangement, VHCDR3 diversity, and somatic mutations to characterize human neonate repertoire. Most of the germline subgroups were identified with frequencies comparable to those present in the adult IgM repertoire except for the IGHV1-2 gene that was preferentially expressed in the cord blood cells. The gene usage diversity contributed to 1,430 unique IGH V-D-J rearrangement patterns while the exonuclease trimming and N region addition at the V-D-J junctions along with gene diversity created a wide range of VHCDR3 with different lengths and sequence variability. We observed a lower degree of somatic mutations in the CDR and framework regions of antibodies from cord blood cells compared to adults. These results provide insights into the characteristics of human cord blood antibody repertoires, which have gene usage diversity and VHCDR3 lengths similar to that of the adult IgM repertoire but differ significantly in some of the gene usages, V-D-J rearrangements, junctional diversity, and somatic mutations.
Starting Page	337
Ending Page	350
Page Count	14
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	64
Issue Number	5
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-12-27
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	454 Sequencing IMGT/HighV-QUEST Antibodyome Human cord blood Antibody repertoire IgM Immunoglobulin Antibody library Human Genetics Allergology Cell Biology Gene Function Immunology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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