NDLI: Molecular characterization and expression analysis of the putative interleukin 6 receptor (IL-6Rα and glycoprotein-130) in rainbow trout (Oncorhynchus mykiss): Salmonid IL-6Rα possesses a polymorphic N-terminal Ig domain with variable numbers of two repeats

Please wait, while we are loading the content...

Application of high-resolution, massively parallel pyrosequencing for estimation of haplotypes and gene expression levels of swine leukocyte antigen (SLA) class I genes

The same ELA class II risk factors confer equine insect bite hypersensitivity in two distinct populations

Atopic dermatitis in West Highland white terriers is associated with a 1.3-Mb region on CFA 17

Toll-like receptor 7 and 8 polymorphisms: associations with functional effects and cellular and antibody responses to measles virus and vaccine

Molecular characterization and expression analysis of the putative interleukin 6 receptor (IL-6Rα and glycoprotein-130) in rainbow trout (Oncorhynchus mykiss): Salmonid IL-6Rα possesses a polymorphic N-terminal Ig domain with variable numbers of two repeats

Position 45 influences the peptide binding motif of HLA-B*44:08

Non-classical MHC-Ι genes in chronic hepatitis B and hepatocellular carcinoma

Erratum to:Dengue virus-specific CD4+ and CD8+ T lymphocytes target NS1, NS3 and NS5 in infected Indian rhesus macaques

Immunogenetics : Volume 64, Issue 2, February 2012

Immunogenetics : Volume 64, Issue 1, January 2012

Immunogenetics : Volume 63

Immunogenetics : Volume 62

Immunogenetics : Volume 61

Immunogenetics : Volume 60

Immunogenetics : Volume 59

Immunogenetics : Volume 58

Immunogenetics : Volume 57

Immunogenetics : Volume 56

Immunogenetics : Volume 55

Immunogenetics : Volume 54

Immunogenetics : Volume 53

Immunogenetics : Volume 52

Immunogenetics : Volume 51

Immunogenetics : Volume 50

Immunogenetics : Volume 49

Immunogenetics : Volume 48

Immunogenetics : Volume 47

Immunogenetics : Volume 46

Immunogenetics : Volume 45

Molecular characterization and expression analysis of the putative interleukin 6 receptor (IL-6Rα and glycoprotein-130) in rainbow trout (Oncorhynchus mykiss): Salmonid IL-6Rα possesses a polymorphic N-terminal Ig domain with variable numbers of two repeats

Content Provider	Springer Nature Link
Author	Costa, Maria M. Wang, Tiehui Monte, Milena M. Secombes, Christopher J.
Copyright Year	2011
Abstract	Interleukin (IL)-6, the founding member of IL-6 family cytokines, plays non-redundant roles in hematopoiesis and acute phase responses. IL-6 signals via a specific private IL-6Rα and a common beta chain gp130. In this study, we have cloned both the IL-6Rα and gp130 in rainbow trout. The trout gp130 cDNA encodes 906 aa and is similar in size, extracellular domain structure (D1–D6) and presence of intracellular motifs important for signal transduction to tetrapod gp130s. The trout IL-6Rα cDNA encodes for 834 aa and is larger compared to tetrapod IL-6Rαs, as are other fish IL-6Rα molecules due to a large D1 domain. However, the cytokine-binding domain is well conserved across vertebrates, with four conserved cysteine residues in the N-terminal FNIII domain and a WSXWS motif in the C-terminal FNIII domain. Furthermore, a phylogenetic tree analysis confirmed that the reported fish IL-6Rα and gp130 molecules are orthologues to their tetrapod counterparts. The extra large D1 domain of the salmonid IL-6Rα molecules results partially from the insertions of two repetitive sequences of [TS]-[TF]-VSTTT-[ND]-TTSNG and TTVS-[AT]-IKD-[DG]-S-[KD]-N-[GR], respectively. Furthermore the numbers of repetitions of the two motifs were variable in different individuals and cell lines, and even in the same fish allelic polymorphism exists. Trout IL-6Rα was expressed at higher levels than gp130 in a number of tissues examined and the expression of both IL-6Rα and gp130 could be modulated by LPS and Poly I:C in the cell lines studied. The expression patterns of the receptors suggest that high level expression of IL-6Rα is critical for IL-6 responsiveness.
Starting Page	229
Ending Page	244
Page Count	16
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	64
Issue Number	3
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-10-29
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Interleukin-6 Interleukin-6Ralpha gp130 Expression Phylogenetic analysis Repeat number polymorphism Gene Function Human Genetics Immunology Allergology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Two copies of the genes encoding the subunits of putative interleukin (IL)-4/IL-13 receptors, IL-4Rα, IL-13Rα1 and IL-13Rα2, have been identified in rainbow trout (Oncorhynchus mykiss) and have complex patterns of expression and modulation

Identification of the salmonid IL-17A/F1a/b, IL-17A/F2b, IL-17A/F3 and IL-17N genes and analysis of their expression following in vitro stimulation and infection

Characterization and expression of gamma-interferon-inducible lysosomal thiol reductase (GILT) gene in rainbow trout (Oncorhynchus mykiss) with implications for GILT in innate immune response

Characterization of Toll-like receptor 3 gene in rainbow trout (Oncorhynchus mykiss)

Specific binding of salmonid growth hormone in rainbow trout (Oncorhynchus mykiss) ovary

Cloning, sequencing, and analysis of expression of a second IL-1 β gene in rainbow trout (Oncorhynchus mykiss)

Oncogenic deletion mutants of gp130 signal from intracellular compartments

Rainbow trout (Oncorhynchus mykiss) muscle satellite cells are targets of salmonid alphavirus infection

Two new Ig VH gene families in Oncorhynchus mykiss

Molecular characterization and expression analysis of the putative interleukin 6 receptor (IL-6Rα and glycoprotein-130) in rainbow trout (Oncorhynchus mykiss): Salmonid IL-6Rα possesses a polymorphic N-terminal Ig domain with variable numbers of two repeats

Similar Documents

Two copies of the genes encoding the subunits of putative interleukin (IL)-4/IL-13 receptors, IL-4Rα, IL-13Rα1 and IL-13Rα2, have been identified in rainbow trout (Oncorhynchus mykiss) and have complex patterns of expression and modulation

Identification of the salmonid IL-17A/F1a/b, IL-17A/F2b, IL-17A/F3 and IL-17N genes and analysis of their expression following in vitro stimulation and infection

Characterization and expression of gamma-interferon-inducible lysosomal thiol reductase (GILT) gene in rainbow trout (Oncorhynchus mykiss) with implications for GILT in innate immune response

Characterization of Toll-like receptor 3 gene in rainbow trout (Oncorhynchus mykiss)

Specific binding of salmonid growth hormone in rainbow trout (Oncorhynchus mykiss) ovary

Cloning, sequencing, and analysis of expression of a second IL-1 β gene in rainbow trout (Oncorhynchus mykiss)

Oncogenic deletion mutants of gp130 signal from intracellular compartments

Rainbow trout (Oncorhynchus mykiss) muscle satellite cells are targets of salmonid alphavirus infection

Two new Ig VH gene families in Oncorhynchus mykiss

Molecular characterization and expression analysis of the putative interleukin 6 receptor (IL-6Rα and glycoprotein-130) in rainbow trout (Oncorhynchus mykiss): Salmonid IL-6Rα possesses a polymorphic N-terminal Ig domain with variable numbers of two repeats