NDLI: Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin

Please wait, while we are loading the content...

Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin

Killer cell immunoglobulin-like receptor and human leukocyte antigen gene profiles in a cohort of HIV-infected Mexican Mestizos

Identification of interleukin genes in Pogona vitticeps using a de novo transcriptome assembly from RNA-seq data

Polymorphism of IL-10 gene promoter region: association with T cell proliferative responses after acellular pertussis vaccination in adults

LILRA6 copy number variation correlates with susceptibility to atopic dermatitis

Immunogenetics : Volume 68, Issue 8, August 2016

Immunogenetics : Volume 68, Issue 6-7, July 2016

Immunogenetics : Volume 68, Issue 5, May 2016

Immunogenetics : Volume 68, Issue 4, April 2016

Immunogenetics : Volume 68, Issue 3, March 2016

Immunogenetics : Volume 68, Issue 2, February 2016

Immunogenetics : Volume 68, Issue 1, January 2016

Immunogenetics : Volume 67

Immunogenetics : Volume 66

Immunogenetics : Volume 65

Immunogenetics : Volume 64

Immunogenetics : Volume 63

Immunogenetics : Volume 62

Immunogenetics : Volume 61

Immunogenetics : Volume 60

Immunogenetics : Volume 59

Immunogenetics : Volume 58

Immunogenetics : Volume 57

Immunogenetics : Volume 56

Immunogenetics : Volume 55

Immunogenetics : Volume 54

Immunogenetics : Volume 53

Immunogenetics : Volume 52

Immunogenetics : Volume 51

Immunogenetics : Volume 50

Immunogenetics : Volume 49

Immunogenetics : Volume 48

Immunogenetics : Volume 47

Immunogenetics : Volume 46

Immunogenetics : Volume 45

Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin

Content Provider	Springer Nature Link
Author	Toh, Masari Shinkai, Hiroki Toki, Daisuke Okumura, Naohiko Tajima, Kiyoshi Uenishi, Hirohide
Copyright Year	2016
Abstract	The nucleotide-binding domain, leucine-rich-containing family, pyrin-domain containing-3 (NLRP3) inflammasome comprises the major components caspase-1, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and NLRP3. NLRP3 plays important roles in maintaining immune homeostasis mediated by intestinal microorganisms and in the immunostimulatory properties of vaccine adjuvants used to induce an immune response. In the present study, we first cloned a complementary DNA (cDNA) encoding porcine ASC because its genomic sequence was not completely determined. The availability of the ASC cDNA enabled us to reconstitute porcine NLRP3 inflammasomes using an in vitro system that led to the identification of the immune functions of porcine NLRP3 and ASC based on the production of interleukin-1β (IL-1β). Further, we identified six synonymous and six nonsynonymous single-nucleotide polymorphisms (SNPs) in the coding sequence of NLRP3 of six breeds of pigs, including major commercial breeds. Among the nonsynonymous SNPs, the Q969R polymorphism is associated with an increased release of IL-1β compared with other porcine NLRP3 variants, indicating that this polymorphism represents a gain-of-function mutation. This allele was detected in 100 % of the analyzed Chinese Jinhua and Japanese wild boars, suggesting that the allele is maintained in the major commercial native European breeds Landrace, Large White, and Berkshire. These findings represent an important contribution to our knowledge of the diversity of NLRP3 nucleotide sequences among various pig populations. Moreover, efforts to exploit the gain of function induced by the Q969R polymorphism promise to improve pig breeding and husbandry by conferring enhanced resistance to pathogens as well as contributing to vaccine efficacy.
Starting Page	693
Ending Page	701
Page Count	9
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	68
Issue Number	9
e-ISSN	14321211
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-05-29
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Innate immunity Single-nucleotide polymorphism Nucleotide-binding domain, leucine-rich-containing family, pyrin-domain containing-3 Pattern recognition receptor Interleukin-1β Pathogen-associated molecular patterns Immunology Human Genetics Gene Function Cell Biology Allergology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Differences in distribution of single nucleotide polymorphisms among intracellular pattern recognition receptors in pigs

Discovery and characterization of small-molecule inhibitors of NLRP3 and NLRC4 inflammasomes.

Activation of NLRP3 Inflammasome and Onset of Alzheimer’s Disease

Cutting Edge: CATERPILLER: A Large Family of Mammalian Genes Containing CARD, Pyrin, Nucleotide-Binding, and Leucine-Rich Repeat Domains

NLRP3 Sensing of Diverse Inflammatory Stimuli Requires Distinct Structural Features

Effect of interleukin-31 on septic shock through regulating inflammasomes and interleukin-1β.

IFN-β: A Contentious Player in Host–Pathogen Interaction in Tuberculosis

A two-nucleotide deletion renders the mannose-binding lectin 2 (MBL2) gene nonfunctional in Danish Landrace and Duroc pigs

The leucine-rich repeat (LRR) domain of NLRP3 is required for NLRP3 inflammasome activation in macrophages.

Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin

Similar Documents

Differences in distribution of single nucleotide polymorphisms among intracellular pattern recognition receptors in pigs

Discovery and characterization of small-molecule inhibitors of NLRP3 and NLRC4 inflammasomes.

Activation of NLRP3 Inflammasome and Onset of Alzheimer’s Disease

Cutting Edge: CATERPILLER: A Large Family of Mammalian Genes Containing CARD, Pyrin, Nucleotide-Binding, and Leucine-Rich Repeat Domains

NLRP3 Sensing of Diverse Inflammatory Stimuli Requires Distinct Structural Features

Effect of interleukin-31 on septic shock through regulating inflammasomes and interleukin-1β.

IFN-β: A Contentious Player in Host–Pathogen Interaction in Tuberculosis

A two-nucleotide deletion renders the mannose-binding lectin 2 (MBL2) gene nonfunctional in Danish Landrace and Duroc pigs

The leucine-rich repeat (LRR) domain of NLRP3 is required for NLRP3 inflammasome activation in macrophages.

Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin