NDLI: Role of the interferon-stimulated response element in HLA-B downregulation in human melanoma cell lines

Content Provider	Springer Nature Link
Author	Griffioen, Marieke Ouwerkerk, Ilse J. M. Harten, Veronique Gobin, Sam J. P. van den Elsen, Peter J. Schrier, P. I.
Copyright Year	1999
Abstract	Tumor cells are thought to escape immune surveillance from T cells by suppressing expression of major histocompatibility complex (MHC) class I molecules at their cell surface. Human MHC class I molecules are encoded by three different loci (HLA-A, -B, and -C). In primary human melanomas as well as melanoma cell lines, HLA class I expression is frequently downregulated in a B locus-specific manner. To study the involvement of promoter elements in HLA-B locus-specific downregulation, a series of reporter constructs containing 5′-flanking sequences of the HLA-A2 and -B7 genes were transfected into melanoma cell lines expressing high and low levels of HLA-B antigens. It is shown that enhancer A, which is generally believed to be a potent enhancer in HLA class I gene transcription, only weakly activates transcription in melanoma cell lines. In contrast, the interferon-stimulated response element (ISRE), known to induce MHC class I expression in response to IFNs, as well as a region comprising site α/enhancer B significantly stimulate constitutive transcription of HLA class I genes. Although none of the promoter elements tested could be demonstrated to mediate HLA-B locus-specific downregulation, high and low HLA-B melanoma cell lines do differ in ISRE activity as well as in ISRE-binding nuclear factors. The finding that high and low HLA-B melanoma cell lines contain different transcription factors binding to elements not actively involved in the process of HLA-B locus abrogation suggests that these cell lines originate from distinct types of melanocyte precursor cells expressing a different set of transcription factors.
Starting Page	287
Ending Page	294
Page Count	8
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	49
Issue Number	4
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-03-03
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

HLA-B locus-specific downregulation in human melanoma requires enhancer A as well as a sequence element located downstream of the transcription initiation site

Patterns of constitutive and IFN-γ inducible expression of HLA class II molecules in human melanoma cell lines

HLA supertype variation across populations: new insights into the role of natural selection in the evolution of HLA-A and HLA-B polymorphisms

Analysis of HLA class I expression in progressing and regressing metastatic melanoma lesions after immunotherapy

Assembly and intracellular trafficking of HLA-B3501 and HLA-B3503

Lack of a Role of the Interferon-stimulated Response Element-like Region in Interferon α-induced Suppression of Hepatitis B Virusin Vitro

Elucidating the origin of HLA-B*73 allelic lineage: Did modern humans benefit by archaic introgression?

Very long haplotype tracts characterized at high resolution from HLA homozygous cell lines

Interferon increases HLA synthesis in melanoma cells: interferon-resistant and -sensitive cell lines.

Role of the interferon-stimulated response element in HLA-B downregulation in human melanoma cell lines

Similar Documents

HLA-B locus-specific downregulation in human melanoma requires enhancer A as well as a sequence element located downstream of the transcription initiation site

Patterns of constitutive and IFN-γ inducible expression of HLA class II molecules in human melanoma cell lines

HLA supertype variation across populations: new insights into the role of natural selection in the evolution of HLA-A and HLA-B polymorphisms

Analysis of HLA class I expression in progressing and regressing metastatic melanoma lesions after immunotherapy

Assembly and intracellular trafficking of HLA-B*3501 and HLA-B*3503

Lack of a Role of the Interferon-stimulated Response Element-like Region in Interferon α-induced Suppression of Hepatitis B Virusin Vitro

Elucidating the origin of HLA-B*73 allelic lineage: Did modern humans benefit by archaic introgression?

Very long haplotype tracts characterized at high resolution from HLA homozygous cell lines

Interferon increases HLA synthesis in melanoma cells: interferon-resistant and -sensitive cell lines.

Role of the interferon-stimulated response element in HLA-B downregulation in human melanoma cell lines

Assembly and intracellular trafficking of HLA-B3501 and HLA-B3503