NDLI: Extracellular domain alterations impact surface expression of stimulatory natural killer cell receptor KIR2DS5

Content Provider	Springer Nature Link
Author	Steiner, riko K. Dakshanamurthy, Sivanesan VandenBussche, Christopher J. Hurley, Carolyn K.
Copyright Year	2008
Abstract	In the human killer cell immunoglobulin-like receptors, KIR2DL2, and KIR2DL3, a triad of amino acids in the D1 domain interact to stabilize protein structure. Substitution of any one of these residues caused significant loss of cell surface expression. Although KIR2DS4 and KIR2DS5, two homologous receptors, differ for this triad, flow cytometry analysis of NK and T cell lines transfected with stimulatory KIR genes KIR2DS4 (allele 001) and KIR2DS5 (allele 002) demonstrated cell surface expression. For KIR2DS5, restoration of the triad sequence increased surface expression. Further studies of the receptor encoded by KIR2DS5002 showed both mature and immature protein isoforms upon gel electrophoresis coupled with surface biotinylation or deglycosylation. In contrast, the KIR2DS5001 allelic product was not expressed on the cell surface of either NK or T cells and exhibited only a single immature isoform upon gel electrophoresis. Site-directed mutagenesis demonstrated that absence of the KIR2DS5*001-encoded protein at the cell surface was imparted primarily by two amino acid polymorphisms in the D2 domain. Analysis using molecular dynamics simulations suggested that the substitution of a proline for a serine at residue 111 or the substitution of a serine for a phenylalanine at residue 164 caused destabilization of the domain structure and intracellular retention. A third polymorphism at residue 174 impacted the level of KIR2DS5 surface expression. This is the first description at a stimulatory KIR locus of the impact of specific amino acid variations on receptor maturation and the level of surface expression.
Starting Page	655
Ending Page	667
Page Count	13
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	60
Issue Number	11
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-08-06
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Natural killer cells Cell surface molecules Human Allergology Cell Biology Gene Function Human Genetics Immunology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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