NDLI: Linkage haplotype for allotypic variants of porcine IgA and IgG subclass genes

Content Provider	Springer Nature Link
Author	Kloep, A. Wertz, N. Mendici, M. Ramsoondar, J. Butler, J. E.
Copyright Year	2012
Abstract	Six putative subclasses of expressed porcine IgG have been described from gene sequences and allotypic variants for five of these have been proposed. We tested this hypothesis by studying the transcription of these 11 variants in outbred hemizygous farm pigs. Since Cγ subclass genes are closely linked, they are most likely inherited as a haplotype. Since hemizygous pigs can only express genes encoded on one chromosome, identifying the expressed genes can indicate which allelic variants are linked as well as testing whether the putative alleles are indeed alleles or separate subclass genes. The procedure for producing B cell knockout pigs has recently been described; our study examines transcripts from the hemizygous parents and offspring generated by this technology. More than 570 Cγ gene clones from hemizygous animals were identified according to subclass and allotype by a combination of clone hybridization and sequencing. IgG3 accounted for 80% in newborn animals but <5% in adults. IgG1 accounted for ~50% of all clones recovered from adults and IgG4 was the least frequently recovered (4%). Results indicate that IgG1b, IgG2a, IgG3, IgG4a, IgG5a, and IgG6a are linked and also linked to IgAa. This comprises a haplotype for domesticated swine. For simplicity, we propose that the current nomenclature for the allotypes of IgG1 be reversed so that all genes in the Cγa–Cαa haplotype are designated “a”.
Starting Page	469
Ending Page	473
Page Count	5
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	64
Issue Number	6
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-02-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Allotype IgG IgA Haplotype Swine Human Genetics Gene Function Allergology Cell Biology Immunology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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