NDLI: Characterization and expression analysis of CD3ɛ and CD3γ/δ in fugu, Takifugu rubripes

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Characterization and expression analysis of CD3ɛ and CD3γ/δ in fugu, Takifugu rubripes

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Characterization and expression analysis of CD3ɛ and CD3γ/δ in fugu, Takifugu rubripes

Content Provider	Springer Nature Link
Author	Araki, Kyosuke Suetake, Hiroaki Kikuchi, Kiyoshi Suzuki, Yuzuru
Copyright Year	2005
Abstract	CD3 is an essential component of the CD3-TCR complex. In this report, we describe the cloning, characterization, and expression analysis of the CD3ɛ and CD3γ/δ chain genes from fugu, Takifugu rubripes. Two distinct CD3ɛ homologue cDNAs, designated as CD3ɛ-1 and CD3ɛ-2, and a CD3γ/δ homologue cDNA were isolated from the fugu thymus. The deduced amino acid sequences of these cDNAs exhibit conserved essential CD3 chain motifs and overall structures. RT-PCR analysis demonstrated that the CD3ɛ and CD3γ/δ genes were expressed in lymphoid organs (e.g. thymus, head kidney, trunk kidney and spleen), mucosal tissues (gill, skin, and intestine), and peripheral blood leucocytes (PBL). The CD3 and TCRα genes were expressed only in the surface IgM− population, which were separated from PBL using an anti-fugu IgM monoclonal antibody. In addition, in situ hybridization confirmed that CD3-expressing cells were distributed randomly in the head kidney, trunk kidney, and spleen, but in the thymus were restricted to the lymphoid outer zone and epithelioid inner zone only. Collectively, these results suggest that CD3 molecules are useful markers for the identification of T cells in teleost fish. The present study thus provides a critical step in identifying T cells in this model organism.
Starting Page	158
Ending Page	163
Page Count	6
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	57
Issue Number	1-2
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-03-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fugu T cell CD3 Gene expression Lymphoid organs
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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