NDLI: Novel procedures for high-throughput analysis of a frequent insertion-deletion polymorphism in the human T-cell receptor beta locus

Please wait, while we are loading the content...

Pig-tailed macaques (Macaca nemestrina) possess six MHC-E families that are conserved among macaque species: implication for their binding to natural killer receptor variants

Detection of 14 alleles derived from the MHC class I A locus in cynomolgus monkeys

Isolation of monotreme T-cell receptor α and β chains

Bioinformatic discovery and initial characterisation of nine novel antimicrobial peptide genes in the chicken

Gene duplication and gene conversion in class II MHC genes of New Zealand robins (Petroicidae)

The dominant MHC class I gene is adjacent to the polymorphic TAP2 gene in the duck, Anas platyrhynchos

Early expression of two TdT isoforms in the hematopoietic system of the Mexican axolotl : Implications for the evolutionary origin of the N-nucleotide addition

Mutations of the T-cell receptor constant region after in utero stem cell transplantation

Novel procedures for high-throughput analysis of a frequent insertion-deletion polymorphism in the human T-cell receptor beta locus

Immunogenetics : Volume 56, Issue 2, May 2004

Immunogenetics : Volume 56, Issue 1, April 2004

Immunogenetics : Volume 55

Immunogenetics : Volume 54

Immunogenetics : Volume 53

Immunogenetics : Volume 52

Immunogenetics : Volume 51

Immunogenetics : Volume 50

Immunogenetics : Volume 49

Immunogenetics : Volume 48

Immunogenetics : Volume 47

Immunogenetics : Volume 46

Immunogenetics : Volume 45

Novel procedures for high-throughput analysis of a frequent insertion-deletion polymorphism in the human T-cell receptor beta locus

Content Provider	Springer Nature Link
Author	Conrad, Bernard
Copyright Year	2004
Abstract	The human T-cell receptor beta locus (TRB) contains two frequent insertion-deletion polymorphisms. In one, the insertion comprises two functional variable beta genes, TRBV6-2/TRBV6-3 and TRBV4-3, and the pseudogene TRBV3-2. Deletion of these TRBV genes may confer resistance and/or susceptibility to autoimmunity, analogously to findings in rodent models. Curiously, the TRBV domains in the insertion react with the HERV-K18 superantigen associated with type 1 diabetes. While this region has been extensively characterized before, typing methods compatible with high-throughput analysis are not yet available. Here, two novel procedures are reported that are suitable for large-scale association analysis of this polymorphism. One features a duplex TaqMan 5′-exonuclease assay that quantifies the gene dosage of TRBV3-2 present at 0, 1 or 2 copies, with its closely related diploid relative TRBV3-1 as an internal reference, using the 2-ΔΔC T method. The other technique consists of two complementary long PCRs with primers specific for unique regions in the locus. The first discriminates individuals heterozygous or homozygous for the deletion, and the second, individuals heterozygous or homozygous for the insertion from other genotypes. These simple, solid, and cross-validated procedures can now be used in conjunction with flanking single-nucleotide polymorphisms for large-scale linkage studies.
Starting Page	220
Ending Page	224
Page Count	5
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	56
Issue Number	3
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-05-25
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	T-cell receptor beta locus Segmental duplication Genome instability Polymorphism Autoimmunity
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Structural and comparative analysis of the T cell receptor gamma (TRG) locus in Oryctolagus cuniculus

A genetically determined insertion/deletion related polymorphism in human T cell receptor beta chain (TCRB) includes functional variable gene segments

A genetically determined insertion/deletion related polymorphism in human T cell receptor beta chain (TCRB) includes functional variable gene segments

Tetraploid Ancestry Provided Atlantic Salmon With Two Paralogue Functional T Cell Receptor Beta Regions Whereof One Is Completely Novel

Genomic characteristics of the T cell receptor (TRB) locus in the rabbit (Oryctolagus cuniculus) revealed by comparative and phylogenetic analyses

Analysis of Segmental Duplications via Duplication Distance

Structural Variation of the Human Genome

A genetically determined insertion/deletion related polymorphism in human T cell receptor beta chain (TCRB) includes functional variable gene segments

Identification and characterization of novel polymorphic line-1 insertions through comparison of two human genome sequence assemblies (2006).

Novel procedures for high-throughput analysis of a frequent insertion-deletion polymorphism in the human T-cell receptor beta locus

Similar Documents

Structural and comparative analysis of the T cell receptor gamma (TRG) locus in Oryctolagus cuniculus

A genetically determined insertion/deletion related polymorphism in human T cell receptor beta chain (TCRB) includes functional variable gene segments

A genetically determined insertion/deletion related polymorphism in human T cell receptor beta chain (TCRB) includes functional variable gene segments

Tetraploid Ancestry Provided Atlantic Salmon With Two Paralogue Functional T Cell Receptor Beta Regions Whereof One Is Completely Novel

Genomic characteristics of the T cell receptor (TRB) locus in the rabbit (Oryctolagus cuniculus) revealed by comparative and phylogenetic analyses

Analysis of Segmental Duplications via Duplication Distance

Structural Variation of the Human Genome

A genetically determined insertion/deletion related polymorphism in human T cell receptor beta chain (TCRB) includes functional variable gene segments

Identification and characterization of novel polymorphic line-1 insertions through comparison of two human genome sequence assemblies (2006).

Novel procedures for high-throughput analysis of a frequent insertion-deletion polymorphism in the human T-cell receptor beta locus