NDLI: HIV-1–cellular interactions analyzed by single virus tracing

Content Provider	Springer Nature Link
Author	Endreß, Thomas Lampe, Marko Briggs, John A. G. Kräusslich, Hans Georg Bräuchle, Christoph Müller, Barbara Lamb, Don C.
Copyright Year	2008
Abstract	Single virus tracing (SVT) allows the direct investigation of the entry pathway of viruses into living cells. Using fluorescently labeled virus-like particles (VLPs) and SVT, we have studied the interaction between human immunodeficiency virus type 1 (HIV-1) and the plasma membrane of living cells. From the trajectories of freely diffusing VLPs in solution, we established that the particle preparation was homogeneous and the particles had a hydrodynamic radius of 86 ± 5 nm, consistent with the size of single HI viruses. The VLPs that come in contact with the cell surface either become immobilized or rapidly dissociate from the cell surface. The fraction of virions that become immobilized on the plasma membrane correlates with the surface heparan sulfate linked proteoglycans (HSPG) concentration of the cell line tested. The particles that are not immobilized make an average of 1.5 contacts with the cell surface before diffusing away. For most cell lines investigated, the contact duration follows an exponential distribution with a lifetime between 20 and 50 ms depending on the cell type.
Starting Page	1291
Ending Page	1301
Page Count	11
File Format	PDF
ISSN	01757571
Journal	European Biophysics Journal
Volume Number	37
Issue Number	8
e-ISSN	14321017
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-04-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	HIV-1 Heparan sulfate proteoglycans Single virus tracing Fluorescence microscopy Nanotechnology Membrane Biology Neurobiology Cell Biology Biophysics/Biomedical Physics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

HIV-1–cellular interactions analyzed by single virus tracing

Automated multidimensional single molecule fluorescence microscopy feature detection and tracking

Analysis of protein mobilities and interactions in living cells by multifocal fluorescence fluctuation microscopy

Probing elasticity and adhesion of live cells by atomic force microscopy indentation

Optical tweezers to study single Protein A/Immunoglobulin G interactions at varying conditions

Interaction between K+ channel gate modifier hanatoxin and lipid bilayer membranes analyzed by molecular dynamics simulation

Fluorescence photobleaching analysis for the study of cellular dynamics

Structured illumination microscopy of a living cell

Targeting cancer cells: magnetic nanoparticles as drug carriers

HIV-1–cellular interactions analyzed by single virus tracing

Similar Documents

HIV-1–cellular interactions analyzed by single virus tracing

Automated multidimensional single molecule fluorescence microscopy feature detection and tracking

Analysis of protein mobilities and interactions in living cells by multifocal fluorescence fluctuation microscopy

Probing elasticity and adhesion of live cells by atomic force microscopy indentation

Optical tweezers to study single Protein A/Immunoglobulin G interactions at varying conditions

Interaction between K+ channel gate modifier hanatoxin and lipid bilayer membranes analyzed by molecular dynamics simulation

Fluorescence photobleaching analysis for the study of cellular dynamics

Structured illumination microscopy of a living cell

Targeting cancer cells: magnetic nanoparticles as drug carriers

HIV-1–cellular interactions analyzed by single virus tracing