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| Content Provider | Springer Nature Link |
|---|---|
| Author | Schiffler, Burkhard Zöllner, Andy Bernhardt, Rita |
| Copyright Year | 2011 |
| Abstract | In mammals, steroid hormones are synthesized from cholesterol that is metabolized by the mitochondrial CYP11A1 system leading to pregnenolone. The reduction equivalents for this reaction are provided by NADPH, via a small electron transfer chain, consisting of adrenodoxin reductase (AdR) and adrenodoxin (Adx). The reaction partners are involved in a series of transient interactions to realize the electron transfer from NADPH to CYP11A1. Here, we compared the ionic strength effect on the AdR/Adx and Adx/CYP11A1 interactions for wild-type Adx and mutant AdxS112W. Using surface plasmon resonance measurements, stopped flow kinetic investigations and analyses of the product formation, we were able to obtain new insights into the mechanism of these interactions. The replacement of serine 112 by tryptophan was demonstrated to lead to a dramatically decreased k off rate of the Adx/CYP11A1 complex, resulting in a four-fold decreased K d value and indicating a much higher stability of the complex involving the mutant. Stopped flow analysis at various ionic strengths and in different mixing modes revealed that the binding of reduced Adx to CYP11A1 seems to display the limiting step for electron transfer to CYP11A1 with pre-reduced AdxS112W being much more efficient than wild-type Adx. Finally, the dramatic increase in pregnenolone formation at higher ionic strength using the mutant demonstrates that the interaction of CYP11A1 with Adx is the rate-limiting step in substrate conversion and that hydrophobic interactions may considerably improve this interaction and the efficiency of product formation. The data are discussed using published structural data of the complexes. |
| Starting Page | 1275 |
| Ending Page | 1282 |
| Page Count | 8 |
| File Format | |
| ISSN | 01757571 |
| Journal | European Biophysics Journal |
| Volume Number | 40 |
| Issue Number | 12 |
| e-ISSN | 14321017 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2011-04-28 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Adrenodoxin Adrenodoxin reductase CYP11A1 Stopped flow Biacore Mitochondrial steroid hydroxylase system Biochemistry Nanotechnology Biophysics and Biological Physics Membrane Biology Neurobiology Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biophysics |
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