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ANNINE-6plus, a voltage-sensitive dye with good solubility, strong membrane binding and high sensitivity

Content Provider	Springer Nature Link
Author	Fromherz, Peter Hübener, Gerd Kuhn, Bernd Hinner, Marlon J.
Copyright Year	2007
Abstract	We present a novel voltage-sensitive hemicyanine dye ANNINE-6plus and describe its synthesis, its properties and its voltage-sensitivity in neurons. The dye ANNINE-6plus is a salt with a double positively charged chromophore and two bromide counterions. It is derived from the zwitterionic dye ANNINE-6. While ANNINE-6 is insoluble in water, ANNINE-6plus exhibits a high solubility of around 1 mM. Nonetheless, it displays a strong binding to lipid membranes. In contrast to ANNINE-6, the novel dye can be used to stain cells from aqueous solution without surfactants or organic solvents. In neuronal membranes, ANNINE-6plus exhibits the same molecular Stark effect as ANNINE-6. As a consequence, a high voltage-sensitivity is achieved with illumination and detection in the red end of the excitation and emission spectra, respectively. ANNINE-6plus will be particularly useful for sensitive optical recording of neuronal excitation when organic solvents and surfactants must be avoided as with intracellular or extracellular staining of brain tissue.
Starting Page	509
Ending Page	514
Page Count	6
File Format	PDF
ISSN	01757571
Journal	European Biophysics Journal
Volume Number	37
Issue Number	4
e-ISSN	14321017
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-08-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Voltage-sensitive dye Optical recording Fluorescence Membrane voltage Nerve cells Neurobiology Animal Physiology Plant Physiology Biochemistry Cell Biology Biophysics/Biomedical Physics
Content Type	Text
Resource Type	Article
Subject	Medicine Biophysics

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ANNINE-6plus, a voltage-sensitive dye with good solubility, strong membrane binding and high sensitivity

ANNINE-6plus, a voltage-sensitive dye with good solubility, strong membrane binding and high sensitivity

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ANNINE-6plus, a voltage-sensitive dye with good solubility, strong membrane binding and high sensitivity

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