NDLI: Selective condensation of DNA by aminoglycoside antibiotics

Content Provider	Springer Nature Link
Author	Kopaczynska, M. Schulz, A. Fraczkowska, K. Kraszewski, S. Podbielska, H. Fuhrhop, J. H.
Copyright Year	2015
Abstract	The condensing effect of aminoglycoside antibiotics on the structure of double-stranded DNA was examined. The selective condensation of DNA by small molecules is an interesting approach in biotechnology. Here, we present the interaction between calf thymus DNA and three types of antibiotic molecules: tobramycin, kanamycin, and neomycin. Several techniques were applied to study this effect. Atomic force microscopy, transmission electron microscopy images, and nuclear magnetic resonance spectra showed that the interaction of tobramycin with double-stranded DNA caused the rod, toroid, and sphere formation and very strong condensation of DNA strands, which was not observed in the case of other aminoglycosides used in the experiment. Studies on the mechanisms by which small molecules interact with DNA are important in understanding their functioning in cells, in designing new and efficient drugs, or in minimizing their adverse side effects. Specific interactions between tobramycin and DNA double helix was modeled using molecular dynamics simulations. Simulation study shows the aminoglycoside specificity to bend DNA double helix, shedding light on the origins of toroid formation. This phenomenon may lighten the ototoxicity or nephrotoxicity issues, but also other adverse reactions of aminoglycoside antibiotics in the human body.
Starting Page	287
Ending Page	299
Page Count	13
File Format	PDF
ISSN	01757571
Journal	European Biophysics Journal
Volume Number	45
Issue Number	4
e-ISSN	14321017
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-12-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Deoxyribonucleic acid condensation Toroids Aminoglycoside antibiotics Atomic force microscopy Electron microscopy Molecular dynamics Biochemistry Biophysics and Biological Physics Cell Biology Neurobiology Membrane Biology Nanotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Biophysics

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