NDLI: Concentration dependent effect of GsMTx4 on mechanosensitive channels of small conductance in E. coli spheroplasts

Content Provider	Springer Nature Link
Author	Hurst, Annette C. Gottlieb, Philip A. Martinac, Boris
Copyright Year	2008
Abstract	The spider peptide GsMTx4, at saturating concentration of 5 μM, is an effective and specific inhibitor for stretch-activated mechanosensitive (MS) channels found in a variety of eukaryotic cells. Although the structure of the peptide has been solved, the mode of action remains to be determined. Because of its amphipathic structure, the peptide is proposed to interact with lipids at the boundaries of the MS channel proteins. In addition, GsMTx4 has antimicrobial effects, inhibiting growth of several species of bacteria in the range of 5–64 μM. Previous studies on prokaryotic MS channels, which serve as model systems to explore the principle of MS channel gating, have shown that various amphipathic compounds acting at the protein–lipid interface affect MS channel gating. We have therefore analyzed the effect of different concentrations of extracellular GsMTx4 on MS channels of small conductance, MscS and MscK, in the cytoplasmic membrane of wild-type E. coli spheroplasts using the patch-clamp technique. Our study shows that the peptide GsMTx4 exhibits a biphasic response in which peptide concentration determines inhibition or potentiation of activity in prokaryotic MS channels. At low peptide concentrations of 2 and 4 μM the gating of the prokaryotic MS channels was hampered, manifested by a decrease in pressure sensitivity. In contrast, application of peptide at concentrations of 12 and 20 μM facilitated prokaryotic MS channel opening by increasing the pressure sensitivity.
Starting Page	415
Ending Page	425
Page Count	11
File Format	PDF
ISSN	01757571
Journal	European Biophysics Journal
Volume Number	38
Issue Number	4
e-ISSN	14321017
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-01-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	MscS MscK Patch-clamp Grammostola peptide Amphipath Biphasic response Nanotechnology Neurobiology Membrane Biology Cell Biology Biophysics/Biomedical Physics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biophysics

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