NDLI: Depth-sensing analysis of cytoskeleton organization based on AFM data

Content Provider	Springer Nature Link
Author	Pogoda, Katarzyna Jaczewska, Justyna Wiltowska Zuber, Joanna Klymenko, Olesya Zuber, Kazimierz Fornal, Maria Lekka, Małgorzata
Copyright Year	2011
Abstract	Atomic force microscopy is a common technique used to determine the elastic properties of living cells. It furnishes the relative Young’s modulus, which is typically determined for indentation depths within the range 300–500 nm. Here, we present the results of depth-sensing analysis of the mechanical properties of living fibroblasts measured under physiological conditions. Distributions of the Young’s moduli were obtained for all studied cells and for every cell. The results show that for small indentation depths, histograms of the relative values of the Young’s modulus described the regions rich in the network of actin filaments. For large indentation depths, the overall stiffness of a whole cell was obtained, which was accompanied by a decrease of the modulus value. In conclusion, the results enable us to describe the non-homogeneity of the cell cytoskeleton, particularly, its contribution linked to actin filaments located beneath the cell membrane. Preliminary results showing a potential application to improve the detection of cancerous cells, have been presented for melanoma cell lines.
Starting Page	79
Ending Page	87
Page Count	9
File Format	PDF
ISSN	01757571
Journal	European Biophysics Journal
Volume Number	41
Issue Number	1
e-ISSN	14321017
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-10-27
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell biomechanics Atomic force microscopy Depth-sensing analysis Cell cytoskeleton Biophysics and Biological Physics Cell Biology Membrane Biology Biochemistry Nanotechnology Neurobiology
Content Type	Text
Resource Type	Article
Subject	Medicine Biophysics

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