NDLI: O-GlcNAc modification of proteins affects volume regulation in Jurkat cells

Content Provider	Springer Nature Link
Author	Nagy, Tamás Balasa, Alfréd Frank, Dorottya Rab, András Rideg, Orsolya Kotek, Gyula Magyarlaki, Tamás Bogner, Péter Kovács, Gábor L. Miseta, Attila
Copyright Year	2009
Abstract	An increasing amount of recent research has demonstrated that the hexosamine biosynthesis pathway (HBP) plays a significant role in the modulation of intracellular signaling transduction pathways, and affects cellular processes via modification of protein by O-linked β-N-acetylglucosamine (O-GlcNAc). Besides the many known and postulated effects of protein O-GlcNAc modifications, there is little available data on the role of O-GlcNAc in cellular volume regulation. Our objective was to test the effect of increased O-GlcNAc levels on hypotonia-induced volume changes in Jurkat cells. We pretreated Jurkat cells for 1 h with glucosamine (GlcN), PUGNAc (O-(2-acetamido-2-deoxy-d-glucopyranosylidene)-amino-N-phenylcarbamate) an inhibitor of O-GlcNAcase, or a high level of glucose to induce elevated levels of O-GlcNAc. We found that the response of Jurkat cells to hypotonic stress was significantly altered. The hypotonia induced cell-swelling was augmented in both GlcN and PUGNAc-treated cells and, to a lesser extent, in high glucose concentration-treated cells. Evaluated by NMR measurements, GlcN and PUGNAc treatment also significantly reduced intracellular water diffusion. Taken together, increased cell swelling and reduced water diffusion caused by elevated O-GlcNAc show notable analogy to the regulatory volume changes seen by magnetic resonance methods in nervous and other tissues in different pathological states. In conclusion, we demonstrate for the first time that protein O-GlcNAc could modulate cell volume regulation.
Starting Page	1207
Ending Page	1217
Page Count	11
File Format	PDF
ISSN	01757571
Journal	European Biophysics Journal
Volume Number	39
Issue Number	8
e-ISSN	14321017
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-12-31
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	O-GlcNAc Hexosamine biosynthesis pathway Volume regulation Osmotic stress Apparent diffusion coefficient Nanotechnology Neurobiology Membrane Biology Cell Biology Biophysics and Biological Physics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biophysics

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