NDLI: Further analysis of the role of spectrin repeat motifs in α-actinin dimer formation

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Further analysis of the role of spectrin repeat motifs in α-actinin dimer formation

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European Biophysics Journal : Volume 25, Issue 4, March 1997

European Biophysics Journal : Volume 25, Issue 3, January 1997

Further analysis of the role of spectrin repeat motifs in α-actinin dimer formation

Content Provider	Springer Nature Link
Author	Flood, G. Rowe, A. J. Critchley, D. R. Gratzer, W. B.
Copyright Year	1997
Abstract	Protein constructs consisting of repeats 1–4, repeats 1–3 and repeats 2–4 of the rod domain of chicken α-actinin were expressed as fusion proteins in Escherichia coli. Based on the evidence of circular dichroism spectra and cooperative thermal unfolding profiles both truncated rod fragments were judged to have assumed the native structural fold. The thermal stabilities were in both cases significantly lower than that of the intact rod (repeats 1–4). Analyses by sedimentation equilibrium and velocity provided further evidence to show that fragment 1–4 is entirely dimeric in the concentration range of these experiments, resembling therefore the rod domain isolated by proteolytic digestion of native α-actinin. Fragment 2–4, and probably also 1–3, show concentration-dependent association, with dissociation constants, estimated by sedimentation equilibrium, in the 1–10 µM range. Thus, in confirmation of earlier work, all four repeats are re-quired to generate a maximally stable anti-parallel dimer (Kd∼10 pM), suggesting the presence of binding sites in all of them to allow for aligned pairing.
Starting Page	431
Ending Page	435
Page Count	5
File Format	PDF
ISSN	01757571
Journal	European Biophysics Journal
Volume Number	25
Issue Number	5-6
e-ISSN	14321017
Language	English
Publisher	Springer-Verlag
Publisher Date	1997-05-13
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Medicine Biophysics

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Abbreviations: sr- spectrin repeat; (2014).

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