NDLI: Wide applicability of a flow cytometric assay to measure absolute membrane potentials on the millivolt scale

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Wide applicability of a flow cytometric assay to measure absolute membrane potentials on the millivolt scale

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European Biophysics Journal : Volume 27

European Biophysics Journal : Volume 26

European Biophysics Journal : Volume 25

Wide applicability of a flow cytometric assay to measure absolute membrane potentials on the millivolt scale

Content Provider	Springer Nature Link
Author	Emri, Miklós Balkay, László Krasznai, Zoltán Trón, Lajos Márián, Teréz
Copyright Year	1998
Abstract	To prove the general applicability of a recently published flow cytometric method to determine the membrane potentials of cells on the absolute (mV) scale, the validity of the premises involved were analyzed individually. Experimental evidence was given for bis-oxonol, the applied membrane potential indicator being a Nernstian dye. The results of special measurements proved that extracellular dye concentrations were not affected by cellular dye uptake under the applied experimental conditions and that the dye content of the suspending medium did not contribute directly to the measured cellular fluorescence. A direct, membrane-potential-independent contribution of the extracellular dye to cellular fluorescence was also found to be negligible, as membrane potential values of the same type of cells evaluated from measurements in the presence of different extracellular oxonol concentrations were very close to each other. The transmembrane potential of B lymphoid JY cells was measured by the method as a function of cell density in the tissue culture. Cells isolated during the log phase of growth displayed a –40±4 mV membrane potential. At a high density of the culture (plateau phase), a significant increase of the membrane potential to –61±3 mV was observed and a medium value of –47±3.5 mV was measured at an intermediate density of the cells. Our observation indicates that nonadherent cells can also be hyperpolarized when optimal growth conditions are terminated.
Starting Page	78
Ending Page	83
Page Count	6
File Format	PDF
ISSN	01757571
Journal	European Biophysics Journal
Volume Number	28
Issue Number	1
e-ISSN	14321017
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-11-27
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Medicine Biophysics

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