NDLI: The Impact of Oxygen Tension on Cell Density and Metabolic Diversity of Microbial Communities in Alkane Degrading Continuous-Flow Cultures

Content Provider	Springer Nature Link
Author	Berthe Corti, L. Bruns, A.
Copyright Year	1999
Abstract	The impact of the dissolved O2 tension (DOT) and the dilution rate on the metabolic diversity of an autochthonous hexadecane-degrading community in continuous-flow cultures containing hexadecane-coated intertidal sediment was determined in a set of experiments. The DOT was kept constant within each culture at values of 80% (168 μmol O2L−1) or 0.4% (0.84 μmol O2 L−1). The dilution rate was increased from D= 0.012 h−1 to D= 0.06 h−1. To determine the culture activity, we analyzed the hexadecane degradation rate, the protein production rate, and the oxygen consumption rate. The cell concentration of different metabolic groups was determined by colony forming units (CFU), and by most probable number (MPN). The metabolic diversity was determined by the substrate utilization spectrum in Biolog GN microtiter plates. The substrate utilization pattern of the cultures decreased considerably as D increased. This effect was more pronounced at 0.4% of DOT than at 80% of DOT. The MPN and CFU revealed that as D increased, only minor changes occurred in the community structure. The hexadecane degradation rate, the protein production rate, and the oxygen consumption rate increased parallel to D independently of the DOT. This means that the biocenosis at 0.4% of DOT was different from the biocenosis at 80% of DOT, although the metabolic activity of the cultures was unaffected by a 200-factor difference in the oxygen tension and revealed a considerable buffer capacity with respect to changes in DOT.
Starting Page	70
Ending Page	77
Page Count	8
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	37
Issue Number	1
e-ISSN	1432184X
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-03-01
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Microbiology Ecology Microbial Ecology Geoecology/Natural Processes Nature Conservation Water Quality/Water Pollution
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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