NDLI: Isolation and Genetic Analysis of Haloalkaliphilic Bacteriophages in a North American Soda Lake

Content Provider	Springer Nature Link
Author	Sabet, Shereen Chu, Weiping Jiang, Sunny C.
Copyright Year	2006
Abstract	Mono Lake is a meromictic, hypersaline, soda lake that harbors a diverse and abundant microbial community. A previous report documented the high viral abundance in Mono Lake, and pulsed-field gel electrophoresis analysis of viral DNA from lake water samples showed a diverse population based on a broad range of viral genome sizes. To better understand the ecology of bacteriophages and their hosts in this unique environment, water samples were collected between February 2001 and July 2004 for isolation of bacteriophages by using four indigenous bacterial hosts. Plaque assay results showed a differential seasonal expression of cultured bacteriophages. To reveal the diversity of uncultured bacteriophages, viral DNA from lake water samples was used to construct clone libraries. Sequence analysis of viral clones revealed homology to viral as well as bacterial proteins. Furthermore, dot blot DNA hybridization analyses showed that the uncultured viruses are more prevalent during most seasons, whereas the viral isolates (Aφ and φ2) were less prevalent, confirming the belief that uncultured viruses represent the dominant members of the community, whereas cultured isolates represent the minority species.
Starting Page	543
Ending Page	554
Page Count	12
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	51
Issue Number	4
e-ISSN	1432184X
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-05-06
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Nature Conservation Geoecology/Natural Processes Microbiology Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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