NDLI: Comprehensive Molecular Characterization of Bacterial Communities in Feces of Pet Birds Using 16S Marker Sequencing

Content Provider	Springer Nature Link
Author	Garcia Mazcorro, Jose F. Castillo Carranza, Stephany A. Guard, Blake Gomez Vazquez, Jose P. Dowd, Scot E. Brigthsmith, Donald J.
Copyright Year	2016
Abstract	Birds and other animals live and evolve in close contact with millions of microorganisms (microbiota). While the avian microbiota has been well characterized in domestic poultry, the microbiota of other bird species has been less investigated. The aim of this study was to describe the fecal bacterial communities of pet birds. Pooled fecal samples from 22 flocks representing over 150 individual birds of three different species (Melopsittacus undulatus or budgerigars, Nymphicus hollandicus or cockatiels, and Serinus canaria or domestic canaries) were used for analysis using the 16S rRNA gene sequencing in the MiSeq platform (Illumina). Firmicutes was the most abundant phylum (median 88.4 %; range 12.9–98.4 %) followed by other low-abundant phyla such as Proteobacteria (median 2.3 %; 0.1–85.3 %) and Actinobacteria (median 1.7 %; 0–18.3 %). Lactobacillaceae (mostly Lactobacillus spp.) was the most abundant family (median 78.1 %; 1.4–97.5 %), especially in budgerigars and canaries, and it deserves attention because of the ascribed beneficial properties of lactic acid bacteria. Importantly, feces from birds contain intestinal, urinary, and reproductive-associated microbiota thus posing a serious problem to study one anatomical region at a time. Other groups of interest include the family Clostridiaceae that showed very low abundance (overall median <0.1 %) with the exception of two samples from cockatiels (14 and 45.9 %) and one sample from budgerigars (19.9 %). Analysis of UniFrac metrics showed that overall, the microbial communities from the 22 flocks tended to cluster together for each bird species, meaning each species shed distinctive bacterial communities in feces. This descriptive analysis provides insight into the fecal microbiota of pet birds.
Starting Page	224
Ending Page	235
Page Count	12
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	73
Issue Number	1
e-ISSN	1432184X
Language	English
Publisher	Springer US
Publisher Date	2016-08-27
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Microbiota Pet birds 16S rRNA gene PICRUSt Health Microbiology Ecology Microbial Ecology Geoecology/Natural Processes Nature Conservation Water Quality/Water Pollution
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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