NDLI: Listeria monocytogenes Inhibits Serotonin Transporter in Human Intestinal Caco-2 Cells

Content Provider	Springer Nature Link
Author	Latorre, E. Pradilla, A. Chueca, B. Pagán, R. Layunta, E. Alcalde, A. I. Mesonero, J. E.
Copyright Year	2016
Abstract	Listeria monocytogenes is a Gram-positive bacterium that can cause a serious infection. Intestinal microorganisms have been demonstrated to contribute to intestinal physiology not only through immunological responses but also by modulating the intestinal serotonergic system. Serotonin (5-HT) is a neuromodulator that is synthesized in the intestinal epithelium and regulates the whole intestinal physiology. The serotonin transporter (SERT), located in enterocytes, controls intestinal 5-HT availability and therefore serotonin’s effects. Infections caused by L. monocytogenes are well described as being due to the invasion of intestinal epithelial cells; however, the effect of L. monocytogenes on the intestinal epithelium remains unknown. The main aim of this work, therefore, was to study the effect of L. monocytogenes on SERT. Caco2/TC7 cell line was used as an enterocyte-like in vitro model, and SERT functional and molecular expression assays were performed. Our results demonstrate that living L. monocytogenes inhibits serotonin uptake by reducing SERT expression at the brush border membrane. However, neither inactivated L. monocytogenes nor soluble metabolites were able to affect SERT. The results also demonstrate that L. monocytogenes yields TLR2 and TLR10 transcriptional changes in intestinal epithelial cells and suggest that TLR10 is potentially involved in the inhibitory effect observed on SERT. Therefore, L. monocytogenes, through TLR10-mediated SERT inhibition, may induce increased intestinal serotonin availability and potentially contributing to intestinal physiological changes and the initiation of the inflammatory response.
Starting Page	730
Ending Page	739
Page Count	10
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	72
Issue Number	3
e-ISSN	1432184X
Language	English
Publisher	Springer US
Publisher Date	2016-08-03
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	5-HT SERT Intestinal epithelium Listeriosis TLR Microbiology Ecology Microbial Ecology Geoecology/Natural Processes Nature Conservation Water Quality/Water Pollution
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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