NDLI: Structural Shifts of Fecal Microbial Communities in Rats with Acute Rejection after Liver Transplantation

Content Provider	Springer Nature Link
Author	Xie, Yirui Luo, Zhuanbo Li, Zhengfeng Deng, Min Liu, Hao Zhu, Biao Ruan, Bing Li, Lanjuan
Copyright Year	2012
Abstract	Bacterial translocation and the development of sepsis after orthotopic liver transplantation (OLT) may be promoted by immunological damage to the intestinal mucosa or by quantitative and qualitative changes in intestinal microbiota. This study monitored structural shifts of gut microbiota in rats with OLT using PCR-denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (RT-qPCR). RT-qPCR targets six major microorganisms (Domain Bacteria, Bacteroides, Bifidobacteria, Enterobacteriaceae, Lactobacillus and Clostridium leptum subgroup). Isograft, Allograft and Sham model were studied. Bacterial translocation to host organs and plasma endotoxin were determined. Alteration in gut microbiota was associated with the elevation of plasma endotoxin and a higher rate of bacterial translocation (BT) to liver in rats with acute rejection. Dynamic analysis of DGGE fingerprints showed that the gut microbiota structure of animals in the three groups was similar before the operation. But significant alterations in the composition of fecal microbiota in Allograft group were observed at 1 and 2 weeks after the OLT. The acute rejection was accompanied by the shifts of gut microbiota towards members of Bacteroides and Ruminococcus. Results from RT-qPCR indicated that Bacteroides significantly increased at 2 weeks after the OLT, whereas numbers of Bifidobacterium spp. decreased at 1 week and recovered at 2 weeks after the OLT. In summary, our data showed that rats with acute rejection after OLT exhibited significant structure shifts in the gut microbiota which dominant by overgrowth of Bacteroides and Ruminococcus, and these were associated with elevation of plasma endotoxin and higher rate of BT.
Starting Page	546
Ending Page	554
Page Count	9
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	64
Issue Number	2
e-ISSN	1432184X
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-03-21
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Nature Conservation Microbiology Ecology Geoecology/Natural Processes Microbial Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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