NDLI: Application of Molecular Techniques to the Elucidation of the Microbial Community Structure of Antique Paintings

Content Provider	Springer Nature Link
Author	Santos, Antonio Cerrada, Alejandro García, Silvia San Andrés, Margarita Abrusci, Concepción Marquina, Domingo
Copyright Year	2009
Abstract	This paper uses molecular techniques to describe the microstructure and microbiological communities of sixteenth century artwork and their relationships. The microbiological populations, analysed by denaturing gradient gel electrophoresis (DGGE), were highly influenced by the chemical composition of the pictorial layers detected by energy-dispersive X-ray analysis. DGGE revealed that the diversity of microbial communities was lower in pictorial layers composed of pigments with metals, such as Pb, Cu and Hg, than in those found in pictorial layers without such compounds. The number of cultivable microorganisms, mainly fungi and bacteria, was very low in comparison to those found by DGGE, revealing the presence of both cultivable and as-yet-uncultivated (or not viable) species in the samples analysed. Both fungi and bacteria were present in a non-random spatial distribution. Environmental scanning electron microscopy and fluorescent in situ hybridisation analyses revealed that bacterial populations were usually found in close contact with the surface of the pictorial layers, and fungal populations were located on the bacterial biofilm. This work shows, for the first time, the correlation between the diversity of the microbial populations and the chemical composition of the pictorial layers of an artwork.
Starting Page	692
Ending Page	702
Page Count	11
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	58
Issue Number	4
e-ISSN	1432184X
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-07-25
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Nature Conservation Geoecology/Natural Processes Microbial Ecology Ecology Microbiology
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Do Botanical Pesticides Alter the Structure of the Soil Microbial Community?

Land Use Intensity Controls Actinobacterial Community Structure

Microbial Community Structure in Three Deep-Sea Carbonate Crusts

Microbial community responses to atrazine exposure and nutrient availability: Linking degradation capacity to community structure

Changes in Soil Bacterial Community Structure with Increasing Disturbance Frequency

Microbial Community Changes Elicited by Exposure to Cyanobacterial Allelochemicals

Response of Microbial Community Composition and Function to Soil Climate Change

Microbial Community Structure and Dynamics in the Largest Natural French Lake (Lake Bourget)

Shifts in Microbial Community Composition Following Surface Application of Dredged River Sediments

Application of Molecular Techniques to the Elucidation of the Microbial Community Structure of Antique Paintings

Similar Documents

Do Botanical Pesticides Alter the Structure of the Soil Microbial Community?

Land Use Intensity Controls Actinobacterial Community Structure

Microbial Community Structure in Three Deep-Sea Carbonate Crusts

Microbial community responses to atrazine exposure and nutrient availability: Linking degradation capacity to community structure

Changes in Soil Bacterial Community Structure with Increasing Disturbance Frequency

Microbial Community Changes Elicited by Exposure to Cyanobacterial Allelochemicals

Response of Microbial Community Composition and Function to Soil Climate Change

Microbial Community Structure and Dynamics in the Largest Natural French Lake (Lake Bourget)

Shifts in Microbial Community Composition Following Surface Application of Dredged River Sediments

Application of Molecular Techniques to the Elucidation of the Microbial Community Structure of Antique Paintings