NDLI: Response of Induced Perturbation on Replicating β-Proteobacterial Ammonia-Oxidizing Populations in Soil

Content Provider	Springer Nature Link
Author	Nyberg, Karin A. Enwall, Karin Schnürer, Anna Sundh, Ingvar Hallin, Sara
Copyright Year	2011
Abstract	The short-term response of induced perturbation by 4-ethylphenol on β-proteobacterial ammonia oxidizers (β-AOB) was investigated in two soils with initial differences in community structure. The hypotheses were that short-term effects of a disturbance of the AOB community is best monitored by specifically looking at the active populations and that soils with dissimilar active AOB populations would display different degree of resistance or resilience. Two soils from a previously characterized long-term field study fertilized with manure or sewage sludge was used. Soil microcosms were incubated in the laboratory over 15 days. The substrate-induced ammonia oxidation was measured, and the composition of β-AOB communities was determined by PCR–DGGE of specific β-AOB 16S rRNA gene fragments. Actively replicating members of the β-AOB were distinguished by the use of bromodeoxyuridine (BrdU) immunocapture. This approach demonstrated that only a minor fraction of the total AOB community was active. Exposure to 4-ethylphenol resulted in approximately 90% lowered substrate-induced ammonia oxidation rates in both soils. This activity inhibition was not accompanied by shifts in β-AOB community structure when total β-AOB DNA was studied. By contrast, changes were seen in the DGGE banding pattern of the BrdU-labeled community DNA after 4-ethylphenol addition in the manure-fertilized soil. In the sewage sludge fertilized soil, the banding pattern of the BrdU-labeled β-AOB remained unchanged, but bands were weaker after the disturbance. In conclusion, it was shown that BrdU immunocapture was applicable to detect shifts in community composition among replicating β-AOB populations in soil. However, this was not reflected by the soils’ ammonia oxidation capacity to resist to or recover from the induced perturbation suggesting that rapid population shifts may not influence soil functioning in a short-term perspective.
Starting Page	701
Ending Page	709
Page Count	9
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	63
Issue Number	3
e-ISSN	1432184X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-11-04
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Geoecology/Natural Processes Microbiology Ecology Microbial Ecology Nature Conservation
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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