NDLI: Characterization of Bacterial Community Structure in Rhizosphere Soil of Grain Legumes

Content Provider	Springer Nature Link
Author	Sharma, S. Aneja, M.K. Mayer, J. Munch, J.C. Schloter, M.
Copyright Year	2005
Abstract	Molecular techniques were used to characterize bacterial community structure, diversity (16S rDNA), and activity (16S rRNA) in rhizospheres of three grain legumes: faba beans (Vicia faba L., cv. Scirocco), peas (Pisum sativum L., cv. Duel) and white lupin (Lupinus albus L., cv. Amiga). All plants were grown in the same soil under controlled conditions in a greenhouse and sampled after fruiting. Amplified 16S rDNA and rRNA products (using universal bacterial primers) were resolved by denaturing gradient gel electrophoresis (DGGE). Distinct profiles were observed for the three legumes with most of the bands derived from RNA being a subset of those derived from DNA. Comparing the total bacterial profiles with actinomycete-specific ones (using actinomycete-specific primers) highlighted the dominance of this group in the three rhizospheres. 16S PCR and RT-PCR products were cloned to construct libraries and 100 clones from each library were sequenced. Actinomycetes and proteobacteria dominated the clone libraries with differences in the groups of proteobacteria. Absence of β-subdivision members in pea and γ-subdivision members of proteobacteria in faba bean rhizosphere was observed. Plant-dependent rhizosphere effects were evident from significant differences in the bacterial community structure of the legume rhizospheres under study. The study gives a detailed picture of both residing and „active” bacterial community in the three rhizospheres. The high abundance of actinomycetes in the rhizospheres of mature legumes indicates their possible role in soil enrichment after the legumes are plowed into the soil as biofertilizers.
Starting Page	407
Ending Page	415
Page Count	9
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	49
Issue Number	3
e-ISSN	1432184X
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-07-07
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Ecology Microbiology Geoecology/Natural Processes Nature Conservation
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterization of Bacterial Community Structure on a Weathered Pegmatitic Granite

Soil and Cultivar Type Shape the Bacterial Community in the Potato Rhizosphere

Community Structure of Actively Growing Bacterial Populations in Plant Pathogen Suppressive Soil

Changes in Soil Bacterial Community Structure with Increasing Disturbance Frequency

Bacterial Activity, Community Structure, and Centimeter-Scale Spatial Heterogeneity in Contaminated Soil

Bacterial Community Succession in Natural River Biofilm Assemblages

Bacterial Community Structure of Biofilms on Artificial Surfaces in an Estuary

Characterization of Fungal Community Structure on a Weathered Pegmatitic Granite

Comparison of Rhizosphere Bacterial Communities in Arabidopsis thaliana Mutants for Systemic Acquired Resistance

Characterization of Bacterial Community Structure in Rhizosphere Soil of Grain Legumes

Similar Documents

Characterization of Bacterial Community Structure on a Weathered Pegmatitic Granite

Soil and Cultivar Type Shape the Bacterial Community in the Potato Rhizosphere

Community Structure of Actively Growing Bacterial Populations in Plant Pathogen Suppressive Soil

Changes in Soil Bacterial Community Structure with Increasing Disturbance Frequency

Bacterial Activity, Community Structure, and Centimeter-Scale Spatial Heterogeneity in Contaminated Soil

Bacterial Community Succession in Natural River Biofilm Assemblages

Bacterial Community Structure of Biofilms on Artificial Surfaces in an Estuary

Characterization of Fungal Community Structure on a Weathered Pegmatitic Granite

Comparison of Rhizosphere Bacterial Communities in Arabidopsis thaliana Mutants for Systemic Acquired Resistance

Characterization of Bacterial Community Structure in Rhizosphere Soil of Grain Legumes