NDLI: Comparison of Eukaryotic Phytobenthic Community Composition in a Polluted River by Partial 18S rRNA Gene Cloning and Sequencing

Content Provider	Springer Nature Link
Author	Dorigo, U. Bérard, A. Humbert, J.F.
Abstract	We compared the species composition in phytobenthic communities at different sampling sites in a small French river presenting polluted and unpolluted areas. For each sampling point, the total DNA was extracted and used to construct an 18S rRNA gene clone library after PCR amplification of a ca 400 bp fragment. Phytobenthic community composition was estimated by random sequencing of several clones per library. Most of the sequences corresponded to the Bacillariophyceae and Chlorophyceae groups. By combining phylogenetic and correspondence analyses, we showed that our molecular approach is able to estimate and compare the species composition at different sampling sites in order to assess the environmental impact of xenobiotics on phytobenthic communities. Changes in species composition of these communities were found, but no evident decrease in the diversity. We discuss the significance of these changes with regard to the existing level of pollution and their impact on the functionality of the ecosystem. Our findings suggest that it is now possible to use faster molecular methods (DGGE, ARISA...) to test large numbers of samples in the context of ecotoxicological studies, and thus to assess the impact of pollution in an aquatic ecosystem.
Starting Page	372
Ending Page	380
Page Count	9
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	44
Issue Number	4
e-ISSN	1432184X
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-10-14
Publisher Place	New York, Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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