NDLI: Identification of Acetate- or Methanol-Assimilating Bacteria under Nitrate-Reducing Conditions by Stable-Isotope Probing

Please wait, while we are loading the content...

Salmonella spp., Vibrio spp., Clostridium perfringens, and Plesiomonas shigelloides in Marine and Freshwater Invertebrates from Coastal California Ecosystems

Penicillium Mycobiota in Arctic Subglacial Ice

Environmental Factors that influence the Transition from Lysogenic to Lytic Existence in the ϕHSIC/Listonella pelagia Marine Phage–Host System

The Influence of Tropical Plant Diversity and Composition on Soil Microbial Communities

Assessing the Contamination Potential of Freshly Extracted Escherichia coli Biofilm Cells by Impedancemetry

Resource-Limited Heterotrophic Prokaryote Production and Its Potential Environmental Impact Associated with Mn Nodule Exploitation in the Northeast Equatorial Pacific

Identification of Acetate- or Methanol-Assimilating Bacteria under Nitrate-Reducing Conditions by Stable-Isotope Probing

Effect of Agricultural Management Regime on Burkholderia Community Structure in Soil

Structural and Functional Responses of a Sewage Microbial Community to Dilution-Induced Reductions in Diversity

Quorum Sensing and Phenazines are Involved in Biofilm Formation by Pseudomonas chlororaphis (aureofaciens) Strain 30-84

Effect of Marine Bacterial Isolates on the Growth and Morphology of Axenic Plantlets of the Green Alga Ulva linza

Stability in a Denitrifying Fluidized Bed Reactor

Zinc Phosphate Transformations by the Paxillus involutus/Pine Ectomycorrhizal Association

Influence of Hydrological Pulse on Bacterial Growth and DOC Uptake in a Clear-Water Amazonian Lake

The Community and Phylogenetic Diversity of Biological Soil Crusts in the Colorado Plateau Studied by Molecular Fingerprinting and Intensive Cultivation

Bacterioplankton Growth and Nutrient Use Efficiencies Under Variable Organic Carbon and Inorganic Phosphorus Ratios

Microbial Ecology : Volume 52, Issue 1, July 2006

Microbial Ecology : Volume 51

Microbial Ecology : Volume 50

Microbial Ecology : Volume 49

Microbial Ecology : Volume 48

Microbial Ecology : Volume 47

Microbial Ecology : Volume 46

Microbial Ecology : Volume 45

Microbial Ecology : Volume 44

Microbial Ecology : Volume 43

Microbial Ecology : Volume 42

Microbial Ecology : Volume 41

Microbial Ecology : Volume 40

Microbial Ecology : Volume 39

Microbial Ecology : Volume 38

Microbial Ecology : Volume 37

Microbial Ecology : Volume 36

Microbial Ecology : Volume 35

Microbial Ecology : Volume 34

Microbial Ecology : Volume 33

Identification of Acetate- or Methanol-Assimilating Bacteria under Nitrate-Reducing Conditions by Stable-Isotope Probing

Content Provider	Springer Nature Link
Author	Osaka, Toshifumi Yoshie, Sachiko Tsuneda, Satoshi Hirata, Akira Iwami, rio Inamori, Yuhei
Copyright Year	2006
Abstract	Stable-isotope probing (SIP) was used to identify acetate- or methanol-assimilating bacteria under nitrate-reducing conditions in activated sludge. A sludge sample obtained from wastewater treatment systems was incubated in a denitrifying batch reactor fed with synthetic wastewater containing [13C]acetate or [13C]methanol as the main carbon source and nitrate as the electron acceptor. We analyzed how growth of bacterial populations was stimulated by acetate or methanol as the external carbon source in nitrogen-removal systems. Most of the acetate- or methanol-assimilating bacteria identified by SIP have been known as denitrifiers in wastewater treatment systems. When acetate was used as the carbon source, 16S rRNA gene sequences retrieved from 13C-labeled DNA were closely related to the 16S rRNA genes of Comamonadaceae (e.g., Comamonas and Acidovorax) and Rhodocyclaceae (e.g., Thauera and Dechloromonas) of the Betaproteobacteria, and Rhodobacteraceae (e.g., Paracoccus and Rhodobacter) of the Alphaproteobacteria. When methanol was used as the carbon source, 16S rRNA gene sequences retrieved from 13C-DNA were affiliated with Methylophilaceae (e.g., Methylophilus, Methylobacillus, and Aminomonas) and Hyphomicrobiaceae. Rarefaction curves for clones retrieved from 13C-DNA showed that the diversity levels for methanol-assimilating bacteria were considerably lower than those for acetate-assimilating bacteria. Furthermore, we characterized nitrite reductase genes (nirS and nirK) as functional marker genes for denitrifier communities in acetate- or methanol-assimilating populations and detected the nirS or nirK sequence related to that of some known pure cultures, such as Alcaligenes, Hyphomicrobium, and Thauera. However, most of the nirS or nirK sequences retrieved from 13C-DNA were clustered in some unidentified groups. On the basis of 16S rRNA gene clone libraries retrieved from 13C-DNA, these unidentified nir sequences might be identified by examining the nir gene in candidates for true denitrifiers (e.g., the families Comamonadaceae, Hyphomicrobiaceae, Methylophilaceae, and Rhodobacteraceae).
Starting Page	253
Ending Page	266
Page Count	14
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	52
Issue Number	2
e-ISSN	1432184X
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-08-05
Publisher Place	New York
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Identification of Novel Betaproteobacteria in a Succinate-Assimilating Population in Denitrifying Rice Paddy Soil by Using Stable Isotope Probing

Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions

Identification of Acetate-Assimilating Microorganisms under Methanogenic Conditions in Anoxic Rice Field Soil by Comparative Stable Isotope Probing of RNA▿

Effect of Incubation Conditions on the Enrichment of Pyrene-degrading Bacteria Identified by Stable-isotope Probing in an Aged, PAH-contaminated Soil

Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions

Methodological Considerations for the Use of Stable Isotope Probing in Microbial Ecology

Discovery of lignin-transforming bacteria and enzymes in thermophilic environments using stable isotope probing.

Identification of Culturable Oligotrophic Bacteria within Naturally Occurring Bacterioplankton Communities of the Ligurian Sea by 16S rRNA Sequencing and Probing

Identification of cellulolytic bacteria in soil by stable isotope probing

Identification of Acetate- or Methanol-Assimilating Bacteria under Nitrate-Reducing Conditions by Stable-Isotope Probing

Similar Documents

Identification of Novel Betaproteobacteria in a Succinate-Assimilating Population in Denitrifying Rice Paddy Soil by Using Stable Isotope Probing

Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions

Identification of Acetate-Assimilating Microorganisms under Methanogenic Conditions in Anoxic Rice Field Soil by Comparative Stable Isotope Probing of RNA▿

Effect of Incubation Conditions on the Enrichment of Pyrene-degrading Bacteria Identified by Stable-isotope Probing in an Aged, PAH-contaminated Soil

Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions

Methodological Considerations for the Use of Stable Isotope Probing in Microbial Ecology

Discovery of lignin-transforming bacteria and enzymes in thermophilic environments using stable isotope probing.

Identification of Culturable Oligotrophic Bacteria within Naturally Occurring Bacterioplankton Communities of the Ligurian Sea by 16S rRNA Sequencing and Probing

Identification of cellulolytic bacteria in soil by stable isotope probing

Identification of Acetate- or Methanol-Assimilating Bacteria under Nitrate-Reducing Conditions by Stable-Isotope Probing