NDLI: Bacterial Community Structure of Autotrophic Denitrification Biocathode by 454 Pyrosequencing of the 16S rRNA Gene

Content Provider	Springer Nature Link
Author	Xiao, Yong Zheng, Yue Wu, Song Yang, Zhao Hui Zhao, Feng
Copyright Year	2014
Abstract	Few studies have been conducted to explore the community composition in denitrifying biocathode. Herein, the microbial communities of denitrifying biocathodes yielding current of 1 mA (reactor C1) and 1.5 mA (reactor C2) were characterized by 454 pyrosequencing. The nitrate removal efficiencies in C1 and C2 were about 93 and 85 %, respectively. The optimization of data generated high-quality sequences of 18509 in C1 and 14857 in C2. Proteobacteria was the predominant phylum, and Bacteroidetes, Chloroflexi, and Planctomycetes were the subdominant groups. Classes of Alphaproteobacteria, Anaerolineae, and Phycisphaerae may benefit the performance of current production and nitrate removal. Twenty-nine dominant operational taxonomic units (OTUs) accounted for 64 and 65 % of sequences in C1 and C2, respectively. A denitrifying pathway was constructed based on the phylogenetic analysis and function inferring of the dominant OTUs. Obviously, the 454 pyrosequencing provided a high-resolution profile of bacteria community in denitrifying biocathode.
Starting Page	492
Ending Page	499
Page Count	8
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	69
Issue Number	3
e-ISSN	1432184X
Language	English
Publisher	Springer US
Publisher Date	2014-09-12
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Microbiology Ecology Microbial Ecology Geoecology/Natural Processes Nature Conservation Water Quality/Water Pollution
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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