NDLI: High-Throughput Sequencing Reveals Drastic Changes in Fungal Communities in the Phyllosphere of Norway Spruce (Picea abies) Following Invasion of the Spruce Bud Scale (Physokermes piceae)

Content Provider	Springer Nature Link
Author	Menkis, Audrius Marčiulynas, Adas Gedminas, Artūras Lynikienė, Jūratė Povilaitienė, Aistė
Copyright Year	2015
Abstract	The aim of this study was to assess the diversity and composition of fungal communities in damaged and undamaged shoots of Norway spruce (Picea abies) following recent invasion of the spruce bud scale (Physokermes piceae) in Lithuania. Sampling was done in July 2013 and included 50 random lateral shoots from ten random trees in each of five visually undamaged and five damaged 40–50-year-old pure stands of P. abies. DNA was isolated from 500 individual shoots, subjected to amplification of the internal transcribed spacer of fungal ribosomal DNA (ITS rDNA), barcoded and sequenced. Clustering of 149,426 high-quality sequences resulted in 1193 non-singleton contigs of which 1039 (87.1 %) were fungal. In total, there were 893 fungal taxa in damaged shoots and 608 taxa in undamaged shoots (p < 0.0001). Furthermore, 431 (41.5 %) fungal taxa were exclusively in damaged shoots, 146 (14.0 %) were exclusively in undamaged shoots, and 462 (44.5 %) were common to both types of samples. Correspondence analysis showed that study sites representing damaged and undamaged shoots were separated from each other, indicating that in these fungal communities, these were largely different and, therefore, heavily affected by P. piceae. In conclusion, the results demonstrated that invasive alien tree pests may have a profound effect on fungal mycobiota associated with the phyllosphere of P. abies, and therefore, in addition to their direct negative effect owing physical damage of the tissue, they may also indirectly determine health, sustainability and, ultimately, distribution of the forest tree species.
Starting Page	904
Ending Page	911
Page Count	8
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	70
Issue Number	4
e-ISSN	1432184X
Language	English
Publisher	Springer US
Publisher Date	2015-06-09
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Forest health Pathogens Pest insects Climate change Fungal community Microbiology Ecology Microbial Ecology Geoecology/Natural Processes Nature Conservation Water Quality/Water Pollution
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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High-throughput sequencing reveals drastic changes in fungal communities in the phyllosphere of Norway spruce ( Picea abies ) following invasion of the spruce bud scale ( Physokermes piceae ) Running title : Impact of invasive Physokermes piceae

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