NDLI: Expansion of Cultured Bacterial Diversity by Large-Scale Dilution-to-Extinction Culturing from a Single Seawater Sample

Content Provider	Springer Nature Link
Author	Yang, Seung Jo Kang, Ilnam Cho, Jang Cheon
Copyright Year	2015
Abstract	High-throughput cultivation (HTC) based on a dilution-to-extinction method has been applied broadly to the cultivation of marine bacterial groups, which has often led to the repeated isolation of abundant lineages such as SAR11 and oligotrophic marine gammaproteobacteria (OMG). In this study, to expand the phylogenetic diversity of HTC isolates, we performed a large-scale HTC with a single surface seawater sample collected from the East Sea, the Western Pacific Ocean. Phylogenetic analyses of the 16S rRNA genes from 847 putative pure cultures demonstrated that some isolates were affiliated with not-yet-cultured clades, including the OPB35 and Puniceicoccaceae marine group of Verrucomicrobia and PS1 of Alphaproteobacteria. In addition, numerous strains were obtained from abundant clades, such as SAR11, marine Roseobacter clade, OMG (e.g., SAR92 and OM60), OM43, and SAR116, thereby increasing the size of available culture resources for representative marine bacterial groups. Comparison between the composition of HTC isolates and the bacterial community structure of the seawater sample used for HTC showed that diverse marine bacterial groups exhibited various growth capabilities under our HTC conditions. The growth response of many bacterial groups, however, was clearly different from that observed with conventional plating methods, as exemplified by numerous isolates of the SAR11 clade and Verrucomicrobia. This study showed that a large number of novel bacterial strains could be obtained by an extensive HTC from even a small number of samples.
Starting Page	29
Ending Page	43
Page Count	15
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	71
Issue Number	1
e-ISSN	1432184X
Language	English
Publisher	Springer US
Publisher Date	2015-11-14
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Dilution-to-extinction High-throughput cultivation Pyrosequencing Marine Roseobacter clade SAR11 Verrucomicrobia Microbiology Ecology Microbial Ecology Geoecology/Natural Processes Nature Conservation Water Quality/Water Pollution
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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