NDLI: Persistence of Antibiotic Resistant Vibrio spp. in Shellfish Hatchery Environment

Content Provider	Springer Nature Link
Author	Dubert, Javier Osorio, Carlos R. Prado, Susana Barja, Juan L.
Copyright Year	2015
Abstract	The characterization of antibiotic-resistant vibrios isolated from shellfish aquaculture is necessary to elucidate the potential transfer of resistance and to establish effective strategies against vibriosis. With this aim, we analyzed a collection of bacterial isolates obtained from 15 failed hatchery larval cultures that, for the most part, had been treated experimentally with chloramphenicol to prevent vibriosis. Isolates were obtained during a 2-year study from experimental cultures of five different clam species. Among a total of 121 Vibrio isolates studied, 28 were found to be chloramphenicol resistant, suggesting that the shellfish hatchery had been using a sublethal concentration of the antibiotic. Interestingly, chloramphenicol-resistant vibrios showed also resistance to tetracycline and amoxicillin (group A; n = 19) or to streptomycin (group B; n = 9). Chloramphenicol-resistant vibrios were subjected to a PCR amplification and DNA sequencing of the chloramphenicol acetyltransferase genes (cat), and the same approach was followed to study the tetracycline resistance markers (tet). 16S ribosomal RNA (rRNA) gene sequencing revealed that chloramphenicol-resistant vibrios pertained mostly to the Splendidus clade. Conjugation assays demonstrated that various R-plasmids which harbored the cat II/tet(D) genes and cat III gene in groups A and B respectively, were transferred to E. coli and bivalve pathogenic vibrios. Most interestingly, transconjugants exhibited the antibiotic resistance patterns of the donors, despite having been selected only on the basis of chloramphenicol resistance. This is the first report carried out in a bivalve hatchery elucidating the persistence of resistant vibrios, the mechanisms of antibiotic resistance, and the transfer of different R-plasmids.
Starting Page	851
Ending Page	860
Page Count	10
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	72
Issue Number	4
e-ISSN	1432184X
Language	English
Publisher	Springer US
Publisher Date	2015-11-09
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Vibrio spp. Antibiotic resistance genes R-plasmids Bivalve cultures Shellfish hatchery Microbiology Ecology Microbial Ecology Geoecology/Natural Processes Nature Conservation Water Quality/Water Pollution
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Functional Metagenomics as a Tool for Identification of New Antibiotic Resistance Genes from Natural Environments

Antibiotics, Antibiotic Resistance Genes, and Bacterial Community Composition in Fresh Water Aquaculture Environment in China

Antibiotic Resistance Genes in Freshwater Biofilms May Reflect Influences from High-Intensity Agriculture

Isolation of Pandemic Vibrio parahaemolyticus from UK Water and Shellfish Produce

Dynamics of Seawater Bacterial Communities in a Shellfish Hatchery

Outcomes of Infections of Sea Anemone Aiptasia pallida with Vibrio spp. Pathogenic to Corals

Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs

Multi-site Analysis Reveals Widespread Antibiotic Resistance in the Marine Pathogen Vibrio vulnificus

Antibiotics and Antibiotic Resistance Genes in Sediment of Honghu Lake and East Dongting Lake, China

Persistence of Antibiotic Resistant Vibrio spp. in Shellfish Hatchery Environment

Similar Documents

Functional Metagenomics as a Tool for Identification of New Antibiotic Resistance Genes from Natural Environments

Antibiotics, Antibiotic Resistance Genes, and Bacterial Community Composition in Fresh Water Aquaculture Environment in China

Antibiotic Resistance Genes in Freshwater Biofilms May Reflect Influences from High-Intensity Agriculture

Isolation of Pandemic Vibrio parahaemolyticus from UK Water and Shellfish Produce

Dynamics of Seawater Bacterial Communities in a Shellfish Hatchery

Outcomes of Infections of Sea Anemone Aiptasia pallida with Vibrio spp. Pathogenic to Corals

Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs

Multi-site Analysis Reveals Widespread Antibiotic Resistance in the Marine Pathogen Vibrio vulnificus

Antibiotics and Antibiotic Resistance Genes in Sediment of Honghu Lake and East Dongting Lake, China

Persistence of Antibiotic Resistant Vibrio spp. in Shellfish Hatchery Environment