NDLI: Studies on the Genotoxicity of Endosulfan in Different Tissues of Fresh Water Fish Mystus vittatus Using the Comet Assay

Content Provider	Springer Nature Link
Author	Sharma, Shilpi Nagpure, N. S. Kumar, Ravindra Pandey, Sanjay Srivastava, Satish K. Singh, Poonam J. Mathur, P. K.
Copyright Year	2007
Abstract	Endosulfan, a widely used organochlorine pesticide, is readily bio-accumulative in fishes and can be indirectly harmful to human populations. Limited efforts have been made to study long-term genotoxic effects of endosulfan in different tissues of fish using gentoxicity biomarkers. Therefore, the current investigation was undertaken to detect single-cell DNA strand breaks induced by endosulfan in the fresh water teleost fish Mystus vittatus using the comet assay. The LC50 value of technical grade endosulfan was first determined for the fish species in a semistatic system, and on the basis of the LC50 value, the sublethal and nonlethal concentrations were determined. The DNA damage was measured in gill, kidney, and erythrocytes as the percentage of DNA in comet tails of fish specimens exposed to the sublethal and nonlethal concentrations of endosulfan. In general, significant effects (p < 0.01) from both concentration and time of exposure were observed in exposed fishes. It was found that all the tissues at all concentrations exhibited the highest DNA damage on day 1, after which there was a nonlinear decline in the percentage of tail DNA. The comparison of DNA damage among the tissues at different concentrations could not show the sensitivity of particular tissue to endosulfan. The current study explored the utility of the comet assay for in vivo laboratory studies using fish species to screen the genotoxic potential of chemical agents.
Starting Page	617
Ending Page	623
Page Count	7
File Format	PDF
ISSN	00904341
Journal	Archives of Environmental Contamination and Toxicology
Volume Number	53
Issue Number	4
e-ISSN	14320703
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-08-20
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Comet assay DNA damage Endosulfan Fish Genotoxicity. Terrestrial Pollution Agriculture Ecology Forestry Environment Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Pollution Health, Toxicology and Mutagenesis Medicine Toxicology

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