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| Content Provider | Springer Nature Link |
|---|---|
| Author | Molak, Martyna Ho, Simon Y. W. |
| Copyright Year | 2011 |
| Abstract | The growth of ancient DNA research has offered exceptional opportunities and raised great expectations, but has also presented some considerable challenges. One of the ongoing issues is the impact of post-mortem damage in DNA molecules. Nucleotide alterations and DNA strand breakages lead to a significant decrease in the quantity of DNA molecules of useful length in a sample and to errors in the final DNA sequences obtained. We present a model of age-dependent DNA damage and quantify the influence of that damage on subsequent steps in the sequencing process, including the polymerase chain reaction and cloning. Calculations using our model show that deposition conditions, rather than the age of a sample, have the greatest influence on the level of DNA damage. In turn, this affects the probability of interpreting an erroneous (possessing damage-derived mutations) sequence as being authentic. We also evaluated the effect of post-mortem damage on real data sets using a Bayesian phylogenetic approach. According to our study, damage-derived sequence alterations appear to have little impact on the final DNA sequences. This indicates the effectiveness of current methods for sequence authentication and validation. |
| Starting Page | 244 |
| Ending Page | 255 |
| Page Count | 12 |
| File Format | |
| ISSN | 00222844 |
| Journal | Journal of Molecular Evolution |
| Volume Number | 73 |
| Issue Number | 3-4 |
| e-ISSN | 14321432 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2011-11-20 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Ancient DNA Post-mortem damage Miscoding lesions Depurination Cytosine deamination Thermal age Plant Sciences Microbiology Cell Biology Animal Genetics and Genomics Plant Genetics & Genomics Evolutionary Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Molecular Biology Ecology, Evolution, Behavior and Systematics |
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