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| Content Provider | Springer Nature Link |
|---|---|
| Author | Mihasan, Marius Brandsch, Roderich |
| Copyright Year | 2013 |
| Abstract | The 165-kb megaplasmid pAO1 of Arthrobacter nicotinovorans carries two large gene clusters, one involved in nicotine catabolism (nic-gene cluster) and one in carbohydrate utilization (ch-gene cluster). Here, we propose that both gene clusters were acquired by A. nicotinovorans by horizontal gene transfer mediated by pAO1. Protein–protein blast search showed that none of the published Arthrobacter genomes contains nic-genes, but Rhodococcus opacus carries on its chromosome a nic-gene cluster highly similar to that of pAO1. Analysis of the nic-genes in the two species suggested a recombination event between their nic-gene clusters. Apparently, there was a gene exchange between pAO1, or a precursor plasmid, and a nic-gene cluster of an as yet unidentified Arthrobacter specie or other soil bacterium, possibly related to Rhodococcus, leading to the transfer by pAO1 of this catabolic trait to A. nicotinovorans. Analysis of the pAO1 ch-gene cluster revealed a virtually identical counterpart on the chromosome of Arthrobacter phenanthrenivorans. Moreover, the sequence analysis of the genes flanking the ch-gene cluster suggested that it was acquired by pAO1 by Xer-related site directed recombination and transferred via the plasmid to A. nicotinovorans. The G+C content, the level of sequence identity, gene co-linearity of nic- and ch-gene clusters as well as the signs of recombination events clearly supports the notion of pAO1 and its precursor plasmids as vehicles in HGT among Gram + soil bacteria. |
| Starting Page | 22 |
| Ending Page | 30 |
| Page Count | 9 |
| File Format | |
| ISSN | 00222844 |
| Journal | Journal of Molecular Evolution |
| Volume Number | 77 |
| Issue Number | 1-2 |
| e-ISSN | 14321432 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2013-07-25 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Catabolic plasmids Horizontal gene transfer Site-specific recombination Nicotine catabolism Carbohydrate catabolism Evolutionary Biology Microbiology Plant Sciences Plant Genetics & Genomics Animal Genetics and Genomics Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Molecular Biology Ecology, Evolution, Behavior and Systematics |
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