NDLI: Comparison of meiofaunal diversity by combined morphological and molecular approaches in a shallow Mediterranean sediment

Content Provider	Springer Nature Link
Author	Rzeznik Orignac, Jadwiga Kalenitchenko, Dimitri Mariette, Jérôme Bodiou, Jean Yves Bris, Nadine Derelle, Evelyne
Copyright Year	2017
Abstract	Fast, accurate and thorough assessments of meiofaunal communities are crucial requirements for ecological studies and routine monitoring of ecosystem status. This study scrutinizes the reliability of the molecular approach through a comparison of morphological and molecular inventories of meiofaunal diversity, with a special focus on nematodes. Sediment samples were collected from a reference coastal Mediterranean site. Metabarcoding analysis was performed using a nuclear marker (small subunit 18S ribosomal RNA) and compared to a morphological analysis performed on the same sample-cores. The results from morphological and molecular inventories differed but were complementary. The molecular analysis revealed a remarkable level of diversity (16 phyla) that exceeded the traditional morphological analysis (10 phyla), showing that meiofaunal diversity can greatly exceed current perceptions. The molecular method proved powerful in detecting the presence of soft-bodied predators, such as Platyhelminthes, possibly reflecting preservation bias in morphological approaches. Even if the molecular inventory identified 57.5% of the sampled diversity, surprisingly, it has not revealed the presence of some nematode genera identified through morphological assessment. While the technique is promising, some further developments are required. As the dominant genus Sabatieria was undetected by the molecular approach, despite being present in the Silva database, improving the knowledge of specific primers should be a priority. Additionally, with 77% of nematode OTUs remaining unassigned at genera level, remedy this lower efficiency requires further investigations to provide DNA-sequences of all morphologically identified species.
Starting Page	1
Ending Page	11
Page Count	11
File Format	PDF
ISSN	00253162
Journal	Marine Biology
Volume Number	164
Issue Number	3
e-ISSN	14321793
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2017-02-07
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Marine & Freshwater Sciences Freshwater & Marine Ecology Oceanography Microbiology Zoology
Content Type	Text
Resource Type	Article
Subject	Ecology Aquatic Science Ecology, Evolution, Behavior and Systematics

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