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| Content Provider | Springer Nature Link |
|---|---|
| Author | Li, Guohua Zhu, Liang He, Yonghong Tan, Hui Sun, Shuqing |
| Copyright Year | 2017 |
| Abstract | A new analytical method has been developed to detect three kinds of DNA simultaneously based on magnetic beads and color-encoded plasmonic nanocrystals. Magnetic beads modified with capture DNA are employed to collect the specific target DNA, and color-encoded plasmonic nanocrystals are applied to signal the target through DNA hybridization. As a proof of concept, three types of representative metal nanocrystals of gold nanoparticle (AuNP), gold nanorod (AuNR), and gold/silver nanoparticle (Au/AgNP) were employed to signal three dissimilar virus-related protective antigen genes, Ebola virus (EV), Variola virus (VV), and Bacillus anthracis (BA), respectively. Detection limits of 0.5–3 fM were obtained showing the high sensitivity for DNA detection. The microscopic discrimination of the encoded nanoparticles allows simple, rapid, accurate, and cost-effective detection of multiple DNA molecules, indicative of the potential in practical applications. Graphical abstract Development of a novel digital triplex DNA assay based on single-countable color-encoded plasmonic nanocrystals |
| Starting Page | 3657 |
| Ending Page | 3666 |
| Page Count | 10 |
| File Format | |
| ISSN | 16182642 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume Number | 409 |
| Issue Number | 14 |
| e-ISSN | 16182650 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2017-03-22 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | DNA assay Plasmonic nanocrystals Color coding Single particle detection Analytical Chemistry Biochemistry Laboratory Medicine Characterization and Evaluation of Materials Food Science Monitoring/Environmental Analysis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Biochemistry |
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