NDLI: Peptide-based fluorescence biosensors for detection/measurement of nanoparticles

Content Provider	Springer Nature Link
Author	Akinloye, Oluyemi Krishnamurthy, Ramanarayan Wishart, David Goss, Greg G.
Copyright Year	2016
Abstract	The ability to detect and quantify nanoparticles is essential but there is currently no simple, sensitive, and rapid method for the detection of nanomaterials. We have developed a novel peptide-based fluorescence-based biosensor for detection and measurement of negatively charged engineered nanoparticles (ENPs). A peptide biosensor (seven lysine residues linked to a cysteine through a three glycine residue linker) with attached fluorescent probes—fluorescein-5-maleimide (F5M) and tetramethylrhodamine-5-maleimide (TMR5M)—was constructed. The fluorescent probes allow close monitoring of the molecular interaction of the labeled peptide with ENPs. The ENP–peptide interaction induces the formation of agglomerates that can be detected and measured by changes in the fluorescence intensities of the labeled peptides or/and by differential light scattering. The relative fluorescence intensities of F5M and TMR5M decreased in a concentration-dependent manner on interaction with various types of negatively charged ENPs (ZnO, Fe3O4, CeO, and single-walled carbon nanotubes). Differential light scattering measurements also showed increases in the hydrodynamic size of the complex. The interactions were not affected by the pH of aqueous media, where humic acid (1 μg/mL) quenched the fluorescence intensity of F5M by approximately 25 %, whereas that of TMR5M was completely quenched. Interference by humic acid at lower concentrations was less prevalent. This novel method is a simple, rapid, and inexpensive in situ assay that shows promise as a primary-level testing technique for detection of ENPs in environmental samples. Graphical Abstract Detection of nanomaterials in aqueous solutions using fluorescently-labeled designer peptides
Starting Page	903
Ending Page	915
Page Count	13
File Format	PDF
ISSN	16182642
Journal	Analytical and Bioanalytical Chemistry
Volume Number	409
Issue Number	4
e-ISSN	16182650
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-11-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nanoparticles Peptide Fluorescein Biosensor Detection Measurement Analytical Chemistry Biochemistry Laboratory Medicine Characterization and Evaluation of Materials Food Science Monitoring/Environmental Analysis
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Biochemistry

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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