NDLI: Analysis of self-incompatibility interactions in 30 resynthesized Brassica napus lines. II. Expression of S-locus glycoproteins (SLGs)

Content Provider	Springer Nature Link
Author	Beschorner, M. Heberer, V. Odenbach, W.
Copyright Year	1999
Abstract	Thirty resynthesized Brassica napus lines with defined S-allele constitution and the ancestral B. oleracea and B. campestris lines were used for the analysis of S- locus glycoproteins (SLGs). The aim of this study was to investigate (1) whether the S-specific glycoproteins of the diploid ancestor lines were also expressed in the amphidiploid hybrids and (2) whether the occurrence of SLG bands was correlated with the activity of the respective S-alleles, which had been tested by means of diallele pollination tests in a previous study. Stigma proteins were separated by isoelectric focusing (IEF)-gel electrophoresis, and glycoprotein bands were identified by Western blotting and Con-A/peroxidase reaction. The SLG bands of the B. campestris parent could be detected in all 30 resynthesized B. napus lines. In contrast, B. oleracea SLG bands could only be detected in 12 resynthesized B. napus lines. Only B. napus lines which carried the dominant B. oleracea S-alleles S8 and S29 showed respective SLG bands in all cases. Nine B. napus lines showed only glycoprotein bands of the B. campestris parent, although the biological functioning of the B. oleracea S-alleles was demonstrated by test-pollinations. New SLG bands different from those of the B. oleracea and B. campestris parents occurred in 16 B. napus lines. The expression level of the SLGs in B. napus was not correlated with the self-incompatibility phenotype, not only in the case of recessive S-alleles (S2, S15), but also for dominant alleles (e.g. S14, S32, S45).
Starting Page	885
Ending Page	890
Page Count	6
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	99
Issue Number	5
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-09-22
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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