NDLI: Discovery of nucleotide polymorphisms in the Musa gene pool by Ecotilling

Content Provider	Springer Nature Link
Author	Till, Bradley J. Jankowicz Cieslak, Joanna Sági, László Huynh, Owen A. Utsushi, Hiroe Swennen, Rony Terauchi, Ryohei Mba, Chikelu
Copyright Year	2010
Abstract	Musa (banana and plantain) is an important genus for the global export market and in local markets where it provides staple food for approximately 400 million people. Hybridization and polyploidization of several (sub)species, combined with vegetative propagation and human selection have produced a complex genetic history. We describe the application of the Ecotilling method for the discovery and characterization of nucleotide polymorphisms in diploid and polyploid accessions of Musa. We discovered over 800 novel alleles in 80 accessions. Sequencing and band evaluation shows Ecotilling to be a robust and accurate platform for the discovery of polymorphisms in homologous and homeologous gene targets. In the process of validating the method, we identified two single nucleotide polymorphisms that may be deleterious for the function of a gene putatively important for phototropism. Evaluation of heterozygous polymorphism and haplotype blocks revealed a high level of nucleotide diversity in Musa accessions. We further applied a strategy for the simultaneous discovery of heterozygous and homozygous polymorphisms in diploid accessions to rapidly evaluate nucleotide diversity in accessions of the same genome type. This strategy can be used to develop hypotheses for inheritance patterns of nucleotide polymorphisms within and between genome types. We conclude that Ecotilling is suitable for diversity studies in Musa, that it can be considered for functional genomics studies and as tool in selecting germplasm for traditional and mutation breeding approaches.
Starting Page	1381
Ending Page	1389
Page Count	9
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	121
Issue Number	7
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-06-30
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Banana Ecotilling Enzymatic mismatch cleavage Musa Nucleotide variation Biotechnology Biochemistry Plant Biochemistry Agriculture Plant Genetics & Genomics Plant Breeding/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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